Identifikasi Molekuler Hawar Daun Bakteri (Xanthomonas oryzae pv. oryzae) dan Uji Patogenisitasnya pada Galur-galur Padi Isogenik

Tasliah Tasliah, Mahrup Mahrup, Joko Prasetiyono

Abstract


Identification of Xanthomonas oryzae pv.
oryzae (Xoo) based on molecular analysis has been
introduced just few years ago. This method used some
specific primers for Xoo and can be done quickly. The
purposes of this research were to identify isolate Xoo
originated from five locations in Indonesia and to determine
the level of pathogenicity of these bacteria. Studies were
conducted in the greenhouse and the Molecular Biology
Laboratory of ICABIOGRAD, from 2011 to 2012. Bacterial
isolates were taken from five regions in Indonesia, namely:
West Sumatra, West Java, Central Java, South Sulawesi, and
West Kalimantan. The specific primers of Xoo were
Xoo2967, Xoo80, and Xoo. Results showed that 216 isolates
could be grown to form yellow colored colonies, which
belongs to a criterian for Xoo. Molecular analysis
demonstrated that 189 isolates were Xoo and 27 isolates
were not. Amplification of DNA of the isolates resulted a 337
bp PCR product for primer Xoo2976, 700 bp for primer
Xoo80 and 534 bp for primer Xoo. Pathogenicity tests of the
Xoo isolates showed xa5, Xa7, and Xa21 resistance genes
were still effective againts BLB pathogens originated from
those five regions, with percentage of resistance were 93.57,
77.49, and 85.37%, respectively.


Keywords


Rice; Xanthomonas oryzae pv. oryzae; specifik primers; test of patogenicity.



DOI: http://dx.doi.org/10.21082/jbio.v9n2.2013.p49-57

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