Perkembangan Aplikasi Teknik Kriopreservasi untuk Konservasi dan Mendukung Program Pemuliaan Tanaman

Ika Roostika

Abstract


In the beginning, cryopreservation
technique was primarily used for germplasms
long-term storage as passive collection because cell division
and metabolism process can be stopped at super low
temperature, commonly in liquid nitrogen. The technique is
suitable for vegetative propagated plants and recalcitrant
seeds. Recently, its application is extending for storing many
species and orthodox seeds. In this paper, the development
of cryopreservation application is discussed. In Indonesia,
cryopreservation is being studied but the application of
cryopreservation has been significantly developed abroad.
The application of cryopreservation technique is not only for
preserving passive collections but also for storing active
collections, including to provide plant materials for hybridization,
cellular engineering, genetic transformation, as well
as pathogens eradication or cryotherapy. It is concluded that
cryopreservation plays an important role in conventional and
modern plant breeding program.


Keywords


Cyopreservation; passive collection; active collection; cryotherapy; plant breeding.

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DOI: http://dx.doi.org/10.21082/jbio.v9n1.2013.p39-48

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P-ISSN : 1907-1094
E-ISSN : 2549-1547


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