Seleksi dan Konfirmasi Alel Gen-gen Hd pada Padi Berumur Genjah dan Produktivitas Tinggi Persilangan Code x Nipponbare

Ahmad Dadang, Tasliah Tasliah, Joko Prasetiyono

Abstract


To support the IP 300 program rice varieties
with both early maturity and high productivity are needed.
The objective of the research was to improve those traits in
Code variety using RIL (recombinant inbreed line) methods.
The research was conducted in the year 2009-2011 at the
greenhouse and laboratory of Molecular Biology, Indonesian
Center for Agricultural Biotechnology and Genetic
Resources Research and Development. Materials consisted
of 600 individuals of F2 derived from one of F1 Code x
Nipponbare crossed plants and they were planted up to F4
with seed-to-seed, then confirmed by 8 microsatelite
markers linked to loci of Hd genes. The molecular analysis
showed that 84 of 600 F2 plants produced. The F4 plants have
50% of flowering time shorter than F2 and F3 plants. Two
lines (CdNb_388 and CdNb_270) have higher productivity
compared to Code by the number of productive tillers (20)
and the number of tiller grains (2240 and 1740) and have
closely 50% of 60 days flowering time of Nipponbare. Three
lines of F4 plants (CdNb_270, 364, and 388) were predicted
to have allele of Hd7 gene, and CdNb_472 was predicted to
have alelle of Hd14 gene.


Keywords


Rice; early maturity; microsatellite; Hd gene.

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DOI: http://dx.doi.org/10.21082/jbio.v9n1.2013.p11-18

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P-ISSN : 1907-1094
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