Struktur Populasi Trichogrammatoidea armigera, Parasitoid Telur Helicoverpa armigera, Berdasarkan Analisis RAPD-PCR

Bahagiawati Amir Husin, Damayanti Buchari, Nurindah Nurindah, Habib Rizjaani, Dwinita Wikan Utami, B. Sahari, A. Sari

Abstract


Population Structure of Trichogrammatoidea armigera,
Egg Parasitoid of Helicoverpa armigera Based on RAPDPCR
Analysis. Bahagiawati, Damayanti Buchari, Nurindah,
H. Rizjaani, Dwinita W. Utami, B. Sahari, and A.
Sari. Genetic structures of Trichogrammatoidea armigera
(Hymenoptera: Trichogrammatidae), the egg parasitoid of
Helicoverpa armigera (Lepidoptera: Noctuidae) were studied.
Egg masses of H. armigera were collected from fields of
several locations in West Java and East Java with different
distances among them and two distinct cultural practices,
i.e., monoculture and polyculture. Genetic relationships
among T. armigera populations that emerged from the collected
H. armigera eggs were analysed by the RAPD-PCR
technique using four oligonucleotide primers. The four
primers revealed 55 presumptive polymorphic loci that were
used to estimate the population structures. The estimated
values of Fixation Index (Fst) was 0.16, indicating that there
was a division of the populations into subpopulations. This
Fst value implied the present of reproductive isolation
among the populations that might be due to their low
migration rate (1.3 insect per generation). This low migration
rate indicated the present of low level of gene flow among
the populations. A dendrogram resulted from the NTSYS
analysis indicated that the West Java and East Java populations
of the egg parasitoid had quite wide genetic distances,
while within each of the populations there was a subdivision
of minor populations. This finding has an important implication
on the program to release Trichogramma spp. as a biological
control agent. The release of the parasitoid cannot be
done randomly, because if we pick up a minor population,
the starter or the released population will mate with the
local population and multiply, thus the inundation will fail to
control the target pest.


Keywords


Trichogrammatoidea armigera; egg parasitoid; Helicoverpa armigera; population structure

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DOI: http://dx.doi.org/10.21082/jbio.v2n2.2006.p52-59

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P-ISSN : 1907-1094
E-ISSN : 2549-1547


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