Perbanyakan dan Konservasi In Vitro Plasma Nutfah Talas (Colocasia esculenta (L.) Schoot)

Nurwita Dewi, Bambang S. Purwoko, Ida Hanarida, Agus Purwito, Iswari S. Dewi

Abstract


Taro is a potential source of carbohydrate
for anticipation of climate change. In vitro technology have
not been widely implemented for tuber crops conservation.
Conservation of the crops is mostly conducted in field. Such
conservation is very susceptible to biotic and abiotic stress.
The research consisted of two activities i.e: micropropagation
and conservation. The objectives were to obtain taro in
vitro propagation and conservation method. The trial was
arranged in a factorial design with six replications. Five taro
accessions were used as the first factor for each study. The
second factor in propagation study was propagation medium
i.e: MS; MS + 2.9 μM IAA + 4.4 μM BA and MS + 2.9 μM IAA
+ 22,2 μM BA. Shoot tip from taro sucker was used as
explant. The second factor in conservation study was MS
medium containing mannitol (0, 30, 40, and 50 g/l). Twoleaves
in vitro shoots from micropropagation study was used
as explants. The addition of BA in MS medium + 2.9 μM IAA
increased the number of shoot of taro germplasm. The best
medium for micropropagation of taro germplasm No. 21 and
Talas Jahe is MS + 2,9 μM IAA + 4,4 μM BA, whereas the
best medium for No. 503, Talas Jahe and Lumbu Banten is
MS + 2,9 μM IAA + 22,2 μM BA. Based on data of plant
height, percentage of leaf life and shelf life, MS medium +
manitol 40 g/l was the best medium for taro germplasm
conservation with prolong sub-culture interval.


Keywords


Colocasia esculenta; micropropagation; conservation; in vitro.

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DOI: http://dx.doi.org/10.21082/jbio.v8n3.2012.p105-112

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P-ISSN : 1907-1094
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