Induksi Tunas pada Kotiledon dan Hipokotil Tanaman Jarak Pagar (Jatropha curcas L.) melalui Organogenesis Tak Langsung

Iswari S Dewi, Anggi Nindita, Bambang S. Purwoko, Darda Efendi

Abstract


Propagation through tissue culture of plant
species with rich secondary metabolites such as Jatropha
curcas L. is difficult to obtain. However, once established, it
can be used as one of the alternatives to supply uniform
propagules. The effects of auxin and cytokinin on the
regulation of de novo woody plants shoot development have
been studied through shoot induction, differentiation and
development. The objective of this research was to identify
explant and suitable culture media for in vitro shoot induction
through indirect organogenesis. Factorial experiment
was arranged in a completely randomized design, replicated
20 times. The first factor was explants, i.e. cotyledons and
hypocotyls. The second factor was MS media containing
combination of plant growth regulator IAA (0, 0.05, and 0.1
mg/l) and BAP (0, 1.0, 2.0, 3.0 mg/l). The results of the
experiment showed that the fastest callus initiation was
achieved by MS + IAA 0.1 mg/l, i.e. 9.5 days after explants
were cultured. Shoots with leaves can be induced from both
cotyledons and hypocotyls. However, hypocotyls gave more
shoots and leaves than cotyledons when cultured in MS +
IAA 0.1 mg/l + BAP 3.0 mg/l. Shoots obtain from hypocotyls
and cotyledons were successfully rooted in MS medium
without any growth regulator.


Keywords


Physic nut; organogenesis; IAA; BAP; hypocotyl; cotyledon.

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DOI: http://dx.doi.org/10.21082/jbio.v8n3.2012.p89-96

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