Analisis Gen Selubung Protein Chilli Veinal Mottle Potyvirus dari Beberapa Daerah di Indonesia

Ifa Manzila, Sri H. Hidayat, Ika Mariska, Sriani Sujiprihati

Abstract


Variation on symptoms and virulence was
observed on different isolates of ChiVMV collected from
West Java, Central Java, East Java, South Kalimantan, West
Sumatera and Central Aceh. Research was conducted to
study genetic variation of six ChiVMV isolates based on
sequence analysis of coat protein (CP) gene and amino
acid. Sequence analysis of CP gene showed 87% to 99%
homology among the six isolates with level of variation
ranging from 0.02% to 1.48%. Sequence analysis of amino
acid derived from CP gene showed 85% to 99% homology.
Further analysis on amino acid motives of CP gene indicated
mutation of octapeptide motif, i.e
LSGQVQPQSRQSEMETEVPQVR on ChiVMV CKB and
RMETFGLDGRVGTQEEDTERHT on other ChiVMV isolates.
Other differences was observed on amino acid number 61
and 84 i.e. deletion of MET and mutation of GG to KV on
ChiVMV BL and KR. Phyllogenetic analysis based on
nucleotide and amino acid sequence showed that six
isolates of ChiVMV can be differentiated into three groups.
ChiVMV KR and BL were in the same group with ChiVMV
Pataruman (GeneBank No. access DQ854961), ChiVMV CKB
was in the same group with ChiVMV Cikabayan 2
(GeneBank No. access DQ854960), and ChiVMV TD, ChiVMV
NI and GB ChiVMV were in the same group with ChiVMV
Taiwan (GeneBank No. access DQ854948). Analysis of CP
gene confirmed the occurrence of genetic variation among
ChiVMV isolates although symptom variation is weak.


Keywords


Chilli veinal motlle potyvirus, coat protein gene, phyllogenetic analysis.

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DOI: http://dx.doi.org/10.21082/jbio.v8n1.2012.p27-37

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P-ISSN : 1907-1094
E-ISSN : 2549-1547


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