Indirect Organogenesis and Somatic Embryogenesis of Pineapple Induced by Dichlorophenoxy Acetic Acid

Ika Roostika, Ika Mariska, Nurul Khumaida, Gustaaf A. Wattimena

Abstract


This research aimed to study the effect of 2,4-D,
AdS, and basal media to the regeneration of pineapple
through indirect organogenesis and somatic embryogenesis,
and to study the complete event of somatic embryogenesis.
Callus formation was induced by 21, 41, and 62 μM 2,4-D
with addition of 9 μM TDZ. The non embryogenic calli were
transferred onto 4.65 μM Kn containing medium.
Embryogenic callus formation was induced on MS or Bac
basal media consisted of N-organic compounds with
addition of AdS (0, 0.05 and 0.1 μM). The embryogenic calli
were regenerated on modified MS medium with addition of
0.9 μM IBA, 1.1 μM BA, 0.09 μM GA3 or MS medium
supplemented with 0.018 mM BA. The result proved that the
single auxin of 2,4-D was not enough to induce embryogenic
cells. Therefore the non embryogenic calli were regenerated
through organogenesis. The 21 μM 2,4-D yielded high level of
callus formation (80%), higher fresh weight (0.2 g/explant)
and higher number of shoot (25 shoots/explant in two
months). Embryogenic calli were produced on N-organic
compounds enriched media. The regeneration medium
significantly affected the level of browning, where the MS
medium with addition of 0.018 mM BA yielded lower level of
browning. There was an interaction of embryogenic callus
induction medium and regeneration medium to the number
of mature somatic embryos. The embryogenic callus
induction on MS medium enriched with N-organic
compounds and 0.05 μM AdS followed by the regeneration
of somatic embryos on MS medium with addition of 0.018
mM BA was the best treatment which yielded 17 mature
somatic embryos/explant.


Keywords


Organogenesis; somatic embryogenesis; pineapple; 2,4-D; adenine sulphate.

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DOI: http://dx.doi.org/10.21082/jbio.v8n1.2012.p8-18

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P-ISSN : 1907-1094
E-ISSN : 2549-1547


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