Penentuan Alergenisitas Protein Gen RB pada Kentang Produk Rekayasa Genetika Berdasarkan Studi Bioinformatika

Eny Ida Riyanti, Edy Listanto, A. Dinar Ambarwati

Abstract


Genetically modified products (GMP) of Katahdin potato event SP951 containing RB gene resistant to late blight diseases
caused by Phytophtora infestans has been developed in the USA. This Katahdin SP951 potato has been crossed with local
varieties Atlantic and Granola for its development in Indonesia. In the release process, the GMP potato should be tested for
environmental and food safety. One of the food safety assessment needs to be done by determining allergenicity of RB protein
whether it is potential as allergen. This research aims to translate the RB gene sequence into RB protein sequence and
investigate the potential RB protein as an allergen through bioinformatic studies. This study was performed based on the
alignment with available protein allergens from available database websites. The predicted RB protein obtained from 2,913
amino acids RB gene was a 971 amino acids length protein with ATG as a start codon and TAA as a stop codon. Bioinformatics
studies of RB protein were performed using www.allergenonline.com, consisted of three searches, i.e. full-length search by
FASTA, 80 amino acids search by FASTA, and 8 amino acid exact matches. For full-length alignment search, there are three
allergen proteins similar with RB protein sequence with the percentage identity of <35%, while for alignment with 80 amino
acids and 8 amino acids did not show similarity with any allergen protein in the database. It can be concluded that RB protein
did not have any potential as an allergen, as according to Codex Alimentarius guidelines for full-length alignment search, only
protein with identity greater than >50% indicating possible cross reactivity with protein allergen.


Keywords


Allergenicity; RB gene; bioinformatic.

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DOI: http://dx.doi.org/10.21082/jbio.v11n3.2015.p81-86

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P-ISSN : 1907-1094
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