Resistance and Phenotypic Character of Chili M2 Mutant Lines Against Chilli Veinal Mottle Virus

Ifa Manzila, Neni Gunaeni, Yenni Kusandriani, Tri P. Priyatno

Abstract


Chilli veinal mottle virus infection (ChiVMV) could reduce the quality and 60–100% of yield losses of chili. Among the chili
varieties released, no one has been resistant to ChiVMV, mainly due to a high variation of ChiVMV strains and not well mapped.
Therefore, finding a new source of ChiVMV resistant genes is pivotal role in order to assembly new varieties. Approach through
in vitro mutation induction using mutagen ethyl methane sulfonate (EMS) is one of the efforts to increase genetic diversity.
Previous studies has successfully acquired 800 M2 lines through callus induction of Gelora variety with EMS. This study aimed to
obtain M2 lines resistant to ChiVMV and having a good agronomical characters. A total of 800 chili M2 lines that derived from chili
M2 mutations using mutagen EMS has been tested in greenhouse to ChiVMV resistance and studied character phenotype. The
results showed that of the 800 lines, there were 28 strains obtained showed a response tolerant and resistant to ChiVMV. Eight
mutant lines of which have good agronomic characters. The mutant lines are M2.100, M2.108, M2.200, M2. 122, M2.238, M2.353,
M2.420, and M2.517. Eight lines will be selected and further observed to obtain chili promising lines that are resistant to ChiVMV
and high yielding.


Keywords


Chili (Capsicum annuum L.); mutation induction; resistanance; ChiVMV.

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DOI: http://dx.doi.org/10.21082/jbio.v11n2.2015.p73-80

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P-ISSN : 1907-1094
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