Analisis Molekuler Gen Partenokarpi DefH9-RI-iaaM pada Progeni Tomat Transgenik

Saptowo J Pardal, Slamet Slamet, Ragapadmi Purnamaningsih, Endang G. Lestari, Sutini Sutini

Abstract


The development of seedless tomato fruits will be more attractive to both consumers and industries. Seedless tomatoes can be
produced through parthenocarpy technology. Artificial parthenocarpy can be induced by conventional crossing, hormone
application, or genetic engineering. The development of parthenocarpic tomatoes through genetic engineering has been carried
out by inserting DefH9-iaaM parthenocarpic geneinto tomato genome via Agrobacterium tumefaciens mediated transformation.
Sixty putative transgenic tomato lines were produced and three events (OvR1#14-4, OvM2#10-1, and OvM2#6-2) were selected
as the best events. The background of the tomato lines was Oval variety, and based on PCR results, the three selected lines
contained DefH9-RI-iaaM in their genome. The objective of this research was to determine the integration of DefH9-RI-iaaM
gene in the progenies of three transgenic tomatoes lines using PCR technique. The research was conducted in the laboratory
and Biosafety Containment Facility of Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and
Development (ICABIOGRAD). Parental variety, Oval (neither transgenic nor in vitro cultured), and elite line of CL 6046 were used
as control plants. The results indicated that the progenies (T1, T2, and T3) of the three tomato lines contained the insert DefH9-RIiaaM
gene.


Keywords


Molecular analysis; transgenic tomato lines; parthenocarpic gene; genetic engineering.

Full Text:

PDF


DOI: http://dx.doi.org/10.21082/jbio.v11n1.2015.p33-40

Refbacks

  • There are currently no refbacks.




Copyright (c) 2016 Jurnal AgroBiogen

 Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.


           


P-ISSN : 1907-1094
E-ISSN : 2549-1547


Jurnal AgroBiogen

Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Jl. Tentara Pelajar 3A, Bogor 16111
Jawa Barat, Indonesia
Telp.: (0251) 8339793, 8337975
Faks.: (0251) 8338820
E-mail: jurnal.agrobiogen@gmail.com
Situs: http://biogen.litbang.pertanian.go.id



View My Stats