ULASAN Masalah Pencoklatan pada Kultur Jaringan

Sri Hutami

Abstract


Several
tropical plant species contain high concentrations of phenolic
compounds, which become oxidised when their cells
are wounded or when the plant parts become senescences.
In tissue culture, the phenolic compounds usually leach into
the medium from the cut surfaces of explants. The phenolic
compounds caused the culture medium turns to dark brown
in colour due to oxidation. This is detrimental to the culture,
because it causes the isolated tissue fails to grow. The
browning of tissue culture and the medium can often be
prevented by one of the several different approaches, such
as by removing the phenolic compounds produced, modifying
the redox potential, inactivating phenolase enzymes,
reducing phenolase activity and substrate availability, as well
as pre-treatments by soaking and preconditioning on a basal
medium.


Keywords


Browning; phenolic compounds; tissue culture.

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DOI: http://dx.doi.org/10.21082/jbio.v4n2.2008.p83-88

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P-ISSN : 1907-1094
E-ISSN : 2549-1547


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