Keragaman Genetik Isolat Cendawan Pyricularia oryzae Menggunakan Primer Pot-2 (Rep-PCR)

Tasliah Tasliah, Reflinur Reflinur, Masdiar Bustamam

Abstract


Rice blast (Pyricularia
oryzae) is one of the most important diseases of rice. It can
be very destructive in the field, when the environmental
conditions are favourable. Information on genetic diversity of
this pathogen could assist plant breeders in determining
strategy for a successful control of the disease. This study
was conducted to analyze genetic diversity in P. oryzae
isolates by a pair of Pot-2 primers using the rep-PCR
technique. These primers were designed from a transposon
element of the entire blast fungus genomic DNA. DNA
samples were extracted from 212 isolates of P. oryzae
collected from two endemic areas of the disease in
Indonesia, i.e., Tamanbogo, Lampung, and Sukabumi, West
Java, as well as from some non-endemic areas in North
Sumatra and West Sumatra). Results of the study indicated
that the 212 isolates could clustered into 21 haplotypes. The
most dominant haplotypes as indicated by their highest
frequency of haplotypes were haplotype Pot 2-019 (54.46%)
followed by haplotype Pot 2-021 (14.73%) and haplotipe Pot
2-016 (6.25%). Regardless of origins of the P. oryzae isolates,
we found 6 haplotypes from Tamanbogo (out of 117
samples), 13 haplotypes from Sukabumi (out of 77 samples),
and 11 haplotypes from North Sumatra and West Sumatra
(out of 18 isolates). It seems that genetic diversity of the P.
oryzae isolates was not affected by the total number of
samples/isolates, but rather by place of the origin and rice
genotypes from which the isolates were collected.


Keywords


Genetic diversity; Pyricularia oryzae; Pot-2 primer; rep-PCR.

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DOI: http://dx.doi.org/10.21082/jbio.v4n2.2008.p70-76

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P-ISSN : 1907-1094
E-ISSN : 2549-1547


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