Pengaruh Sumber Karbon dan Kondisi Inkubasi terhadap Pertumbuhan Kultur In Vitro Purwoceng (Pimpinella pruatjan Molk.)

Ika Roostika, Ragapadmi Purnamaningsih, Arief V. Noviati

Abstract


Pruatjan (Pimpinella pruatjan Molk.) is an Indonesian
medicinal plant which is categorized as endangered
plant and included in Appendix I based on CITES. The in
vitro conservation techniques have been studied. However,
the storage period was very short (4 months) when plant
growth retardant and media dilution were applied. Beside
that, the residual effect of growth retardant was strong
enough so that it needed more than 4 months for recovery.
Thus, the use of certain carbon source may prolong the
preservation period with shorter time for recovery. The
objective of the study was to know the effects of carbon
sources (sucrose and mannitol) and culture conditions (culture
room and growth chamber) to the growth of pruatjan
cultures. This application was hoped to prolong preservation
period of pruatjan longer than 4 months and to cut the
recovery period after presservation. The study was conducted
at Tissue Culture Laboratory in Indonesian Center for
Agricultural Biotechnology and Genetic Resources Research
and Development from August 2006 to July 2007. The
activities included propagation of in vitro shoot grown in
vitro as explants source, preservation of in vitro shoots of
pruatjan, and regeneration of the cultures after preservation.
The experiment was designed as factorial in Randomized
Completely Block Design with 6 replications. The DKW basal
media containing 1 ppm BA, 0.2 ppm thidiazuron, and 100
ppm arginine were supplemented with mannitol or sucrose
at the level of 1, 2, 3, 4, and 5%. The observed variables were
total number of leaves, number of shoot, and number of wilt
leaves. The result revealed that pruatjan cultures could be
stored longer than 4 months. Generally, the effect of
mannitol or sucrose was more dominant than that of cultures
condition. The mannitol (1-5%) strongly inhibited the
growth of pruatjan cultures so that only a few cultures
survived at 7 months preservation period and needed about
1 month for recovery. On the contrary, the effect of sucrose
(at the same level) was better than mannitol. The 2.5%
sucrose optimally inhibited pruatjan cultures. At that condition,
the cultures could be stored for 10 months without
morphological changes so that they could recover spontaneously.


Keywords


Pimpinella pruatjan Molk.; carbon sources; culture conditions.

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DOI: http://dx.doi.org/10.21082/jbio.v4n2.2008.p65-69

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P-ISSN : 1907-1094
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