Analisis Sekuen Gen Tubulin-β Isotipe 1 Cacing Haemonchus contortus Isolat Resisten terhadap Benzimidazole pada Domba di Indonesia

Dyah Haryuningtyas, Wayan T. Artama

Abstract


Benzimidazole (BZ)
resistance to gastrointestinal nematodes in small ruminants
(sheep and goat) has become a significant problem worldwide.
Evidences of anthelmintic resistance to albendazole in
Indonesia has been reported from some government owned
farms in West Java, Central Java, and Yogyakarta. Previous
study on the sheep parasite H. contortus had shown that the
BZ resistance was related to selection for individuals in a
population possesing a spesific β-tubulin isotype 1 gene. The
study is aimed to determine mutation on coding region of
central part of β-tubulin isotype 1 gene of H. contortus resistant
strain from Indonesia. Seven H. contortus worms were
isolated from four BZ resistant sheep from two government
farms (SPTD Trijaya, Kuningan, West Java, and UPTD
Pelayanan Kesehatan Hewan, Bantul, Yogyakarta), and from
a BZ susceptible sheep from Cicurug, Sukabumi, West Java.
DNA was extracted individually from female H. contortus
worms. A fragment of 520 bp β-tubulin isotype 1 gene exon
3, 4, 5 was amplified using the PCR technique and then
sequenced. The results showed that a single mutation
occurred in codon 200 (from phenilalanine to tyrosine) had
caused benzimidazole resistance in H. contortus from SPTD
Trijaya, Kuningan, West Java. Mutation in β-tubulin isotype 1
gene of H. contortus from UPTD Pelayanan Kesehatan
Hewan, Yogyakarta, occurred in codon 198 (from glutamate
to glycine), codon 201 (from cystein to stop codon), and
codon 202 (from isoleucyne to stop codon).


Keywords


Haemonchus contortus; benzimidazole resistance; isotype 1 β-tubulin gene; mutation.

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DOI: http://dx.doi.org/10.21082/jbio.v4n2.2008.p45-50

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P-ISSN : 1907-1094
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