Isolasi Identifikasi Bakteri Penghasil Xilanase serta Karakterisasi Enzimnya

Nur Richana, Tun T. Irawadi, Anwar Nur, Khaswar Syamsu

Abstract


Xylanase is an extracellular enzyme produced by
microorganisms. This enzyme is able to hydrolise xylane
(hemicellulose) to produce xylooligosaccharide and xylose.
Thermoalkaliphilic xylanase is an agent that can be used as
a substitute in the pulp whitening process instead of chlorine.
A study was done to isolate, identificate of bacteria and
characterize xylanase. The isolation of xylanase producing
bacteria has been done from soil and waste of starch industry.
Colonies which produced clearing zone were presumed
as xylanolytic bacteria and chosen for further screening.
Identification of potential isolate in xylanase production was
done using 16S ribosomal RNA sequencing. Isolate Bacillus
pumilus RXA-III5 originated from lime or alkaline soil was
more potential isolate in xylanase production than other 24
isolates. Precipitation of xylanase, that was done using
ammonium sulphate followed by dialyzes produced xylanase
of a higher specific activity (267.1 U.mg-1) than that using
acetone (131.1 U.mg-1) and ethanol (186.65 U.mg-1). Xylanase
was done at purification produced three fractions of xylanase.
Xylanase characteristics consist of pH and temperature
(9 and 50oC), Km and Vmaks value 6 mg.ml-1 and 0.2
mol.minute-1, respectively. The Fe2+ was the strongest activetor
and Mg2+ was the strongest inhibitor activity. This enzyme
was detected as a cellulose-free xylanase. Xylanase is a
prospective agent for bio-bleaching of paper.


Keywords


Isolation; identification; bacteria; xylanase.

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DOI: http://dx.doi.org/10.21082/jbio.v4n1.2008.p24-34

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P-ISSN : 1907-1094
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