Pengujian Nomor-nomor Harapan Padi Tahan Al dan pH Rendah Hasil Seleksi In Vitro dengan Kultur Hara

Ragapadmi Purnamaningsih, Ika Mariska

Abstract


Rice productivity in acid soil is very low because of low pH,
low availability of N, P, K, Ca, Mg, Mo, toxicity of Al and Mn.
Development of Al tolerant varieties could increase rice
productivity in acid soil. Somaclonal variation and in vitro
selection method can be used to develop new Al tolerance
varieties. A rapid screening method is needed to select a
large number of new genotypes or new inbred lines in plant
breeding, such as solution culture methods to evalu-ate Altolerant
rice. This methods was used to know the response
to Al in the seedling stage, root development, and pH
changing. In this experiment solution culture method was
used to evaluate the new genotypes derived from somaclonal
variation and in vitro selection methods. These new
genotypes have been tested the tolerance characteristic by
using AlCl36H2O at 6 concentrations (0, 100, 200, 300, 400,
and 500 ppm). Yoshida solution with two Al concentration
were used to tested these genotypes. Measurement of Al
tolerance was based on root development by using Relative
Root Length (RRL), the relativity of root length at 45 ppm and
0 ppm. Almost all of the genotypes have RRLs higher than
0.7, which means that there was a positive correlation
between the in vitro method and solution culture method. In
this experiment pH changes were not applicable to measure
the tolerance of the rice genotypes to Al and low pH.


Keywords


Rice; aluminum; in vitro selection; somaclonal variation; solution culture testing.

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DOI: http://dx.doi.org/10.21082/jbio.v4n1.2008.p18-23

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P-ISSN : 1907-1094
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