Kriopreservasi untuk Konservasi Plasma Nutfah Tanaman: Peluang Pemanfaatannya di Indonesia

Semuel Leunufna


Increasing rate of plant germplasm lost in
Indonesia has promoted the implementation of various
methods for their conservation. Cryopreservation is a technique
applicable for a long-term preservation (base collection)
of plants possessing non-orthodox (recalcitrant and
semi-recalcitrant) seeds and those propagated vegetatively.
The technique can be used as an alternative method for
orthodox seed plants preservation in the ex situ conservation
system. Although field and in vitro collection methods can
be applied for the non-orthodox seed plants, a number of
disadvantages possesed by these methods, especially in the
tropics or the developing countries, deny their use for the
establishment of a long-term germplasm collection. Successful
implementation of the cryopreservation technique is
supported by the development of protocols, which are able
to provide a high recovery rate for species understudy, using
vitrification based methods which are simple, economical,
applicable to complex organs, and able to implement a high
number of explants per experiment. The availability of infrastructures
including in vitro culture laboratories, continue
supply of liquid nitrogen is highly supporting the use of
cryopreservation technique in Indonesia.


Cryopreservation technique; long term preservation plant germplasm.

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