Regenerasi Pepaya melalui Kultur In Vitro

Diani Damayanti, Sudarsono Sudarsono, Ika Mariska, M. Herman

Abstract


A study was conducted in the Indonesian Center for
Agricultural Biotechnology and Genetic Resources Research
and Development to optimize papaya regeneration systems
through in vitro culture. Four steps were done, i.e., callus
induction, callus regeneration, root formation, and acclimatization.
Explant materials used were immature embryos of
papaya cv. Burung. Immature papaya embryos were cultured
on different media. The best medium for embryogenic
callus development was ½ MS + 10 mg/l 2.4-D + 60% sucrose
+ 143 mg/l adenine sulphate + 50 mg/l myo inositol +
400 mg/l glutamine, while that for callus embryo regeneration
was MS + 0.5 mg/l GA3 + 0.1 mg/l kinetin + Morel and
Wetmore Vitamin. Using this medium, the average of shoot
formation was three shoots per explant of embriogenic
callus, and the percentage of regenerated callus was 80%.
The color of shoot derived from this treatment was green.
Eighty percent of plants formed a complete root development
using ½ MS + 0.5 mg/l paclobutrazol media. Media hull
of rice and compost was the best medium for papaya plant
acclimatization. The percentage of survival on that acclimatization
step was 65%.


Keywords


Papaya; callus induction; callus regeneration; in vitro culture.

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DOI: http://dx.doi.org/10.21082/jbio.v3n2.2007.p49-54

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P-ISSN : 1907-1094
E-ISSN : 2549-1547


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