Regenerasi dan Pertumbuhan Beberapa Varietas Tebu (Saccharum officinarum L.) secara In Vitro

Deden Sukmadjaja, Ade Mulyana

Abstract


(Saccharum officinarum L.) through In Vitro Culture.
Deden Sukmadjaja and Ade Mulyana. The research was
conducted at the Laboratory of Tissue Culture The Biology of
Cell and Tissue Researcher Group ICABIOGRAD, Bogor from
June to November 2009 to studied growth and regenerations
response some varieties of sugarcane through in vitro
culture. The research activities have been carried out in
three steps, i.e., callus formation, regeneration of shoots and
roots regeneration. The type of explants used in the study
was in vitro planlet explants of both sugarcane varieties.
Seven media formulations were used for the callus induction
and regeneration of shoots, while five media formulations
were used for the roots regeneration. The results
showed that the highest respond for calluses induction was
Bulu Lawang varieties at media formulation MS + 2.4-D 2
mg.l-1 + BAP 0.4 mg.l-1 + CH 2000 mg.l-1 and PS 951 varieties
at media formulation MS + 2.4-D 1 mg.l-1 + BAP 0.4 mg.l-1.
While the highest respond for regeneration of shoots was
Bulu Lawang varieties at media formulation MS0 (control
MS) dan PS 951 varieties at media formulation MS + BAP 1
mg.l-1 + kinetin 1 mg.l-1 + NAA 0.5 mg.l-1 + GA3 0.5 mg.l-1.
The highest respond of roots regeneration was Bulu Lawang
and PS 951 varieties at media formulation MS + IBA 1 mg.l-1.
Acclimatization of plantlets produced were grew successfully
about 90-100% in greenhouse.


Keywords


Sugarcane; varieties; growth; regeneration; media formulation

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DOI: http://dx.doi.org/10.21082/jbio.v7n2.2011.p106-118

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