Identifikasi Entomopatogen Bakteri Merah pada Wereng Batang Coklat (Nilaparvata lugens Stål.)

Tri Puji Priyatno, Yohana A Dahliani, Yadi Suryadi, I Made Samudra, Dwi Ningsih Susilowati, Iman Rusmana, Baskoro S Wibowo, Cahyadi Irwan

Abstract


Indentification of Entomopathogenic Red Bacterial from
Brown Planthopper (Nilaparvata lugens Stål.). Tri P.
Priyatno, Yohana A. Dahliani, Yadi Suryadi, I Made
Samudra, Dwi N. Susilowati, Iman Rusmana, Baskoro S.
Wibowo, and Cahyadi Irwan. Red bacteria isolated from
brown planthopper (BPH) has been proven pathogenic
against BPH and others insects. Application of 106 to 107
cells/ml of red bacteria caused 65.6-78.2% mortality of BPH.
The 50% effective concentration (EC50) and lethal time of red
bacteria against BPH is 2.8 x 105 cells/ml and 6.8 days,
respectively. Based on phenotypic characters tested on GN
MicroPlateTM Biolog kit and 16S rRNA sequneces analysis,
red bacteria was identified as Serratia marcescens with 99%
similarity. Red pigmen produced by S. marcescens strain
BPH is secondary metabolite determined as prodigiosin
showing bactericidal activities against Xanthomonas oryzae
pv. oryzae. We concluded that S. marcescens did not only
potent as biocontrol agent to BPH, but also it can be used to
control plant pathogenic bacteria.


Keywords


Entomopathogenic bacteria; Nilaparvata lugens; Serratia marcescens; prodigiosin

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DOI: http://dx.doi.org/10.21082/jbio.v7n2.2011.p85-95

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