Genetic Mapping of SSR Markers in Eight Soybean Chromosomes Based on F2 Population B3462 x B3293

I Made Tasma, Ahmad Warsun, Dani Satyawan, Saptowo Jumali Pardal, Slamet Slamet

Abstract


Genetic Mapping of SSR Markers in Eight Soybean
Chromosomes Based on F2 Population B3462 x B3293. I
Made Tasma, Ahmad Warsun, Dani Satyawan, Saptowo
J. Pardal, and Slamet. Aluminum toxicity is one of the main
contrains for cultivating soybean in acid soils. Genetic
Hak Cipta © 2011, BB-Biogen
mapping of SSR markers is one step for detecting aluminumtoxicity
tolerant QTLs in soybean. Another step is to
phenotype the same population at various aluminum-toxicity
environments. The objectives of this study were to analyze
the segregation of SSR markers in progenies of an F2
population and map the markers in 8 soybean chromosomes.
The F2 population was previously developed by
crossing the Al-tolerant parent B3462 and the Al-sensitive
parent B3293. Polymorphic SSR markers in the parents were
used to PCR amplify DNA of the 100 F2 progenies. PCR
products were separated using agarose or polyacrylamide
gels. A Chi-Square test was done with a null hypothesis that
progenies segregated in a 1 : 2 : 1 ratio. Results showed that
125 SSR markers were polymorphics in the parents. Out of
125 polymorphic markers, 122 were segregated in the
progenies of the F2 population. Among the segregating
markers, 114 were segregated in a 1 : 2 : 1 ratio. Only 8
markers (5.6%) did not follow the 1 : 2 : 1 ratio. One hundred
and nineteen SSR markers were mapped in 8 soybean
chromosomes. These include 18 markers in chromosome
A2, 10 in B1, 16 (C1), 16 (F), 10 (G), 23 (J), 16 (L), and 10 (N).
Total genetic maps covered was 1,194.8 cM with average
map distances between two adjacent markers of 10.7 cM.
Further SSR marker enrichment is required to fill in the gaps
of several chromosomal regions. Genetic maps presented in
this study should be useful for detection of Al-toxicity
tolerant QTLs in soybean.


Keywords


SSR marker; soybean; aluminum toxicity; genetic map; F2 population

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DOI: http://dx.doi.org/10.21082/jbio.v7n2.2011.p69-75

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P-ISSN : 1907-1094
E-ISSN : 2549-1547


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