Peranan Zat Pengatur Tumbuh dalam Perbanyakan Tanaman melalui Kultur Jaringan

Endang Gati Lestari

Abstract


The Role of Growth Regulator in Tissue Culture Plant
Propagation. Endang G. Lestari. In plant tissue culture,
growth regulator has significant roles such as to control root
and shoot development in the plant formation and callus
induction. Cytokinin and auxin are two prominent growth
regulator. Cytokinin consists of BA (benzil adenin), kinetin
(furfuril amino purin), 2-Ip (dimethyl allyl amino purin), and
zeatin. While auksin covers IAA (indone acetic acid), NAA
(napthalene acetic acid), IBA (indole butiric acid) 2.4-D (2.4-
dicholophenoxy acetic acid), dicamba (3,6 dicloro-O-anisic
acid), and picloram (4-amino 3,5,6-tricloropicolinic acid).
The emphasis of plant growth purposes decide the use of
growth regulator. Cytokinin is applied mainly for the purpose
of shoot, while auxin is mainly used for the purpose of root
and callus. The application of growth regulator application is
varied, depending on the genotype and physiological
condition of the plant. The existence of a certain growth
regulating substances can enhance growth regulator activity
of other substances. The type and concentration of the
appropriate growth regulators for each plant is not the same
because it depends on the genotype and physiological
condition of plant tissue. However so often both are
frequently required depend on the ratio/ratio of auxin
cytokines or vice versa. The existence of a certain growth
regulating substances can enhance growth regulator activity
of other substances. The type and concentration of the
appropriate growth regulators for each plant is not the same
because it depends on the genotype and physiological
condition of plant tissue. For the propagation, multiple and
adventive shoots along with embriosomatic formation could
be applied. The seedling is obtained from one somatic cell.
Here, strong auxin, such as dicamba and picloram 2.4-D, is
utilized for callus production. For this reason, seedling per
unit could be produced more than that of organogenesis.


Keywords


Growth regulator; auxin; cytokinin; tissue culture

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DOI: http://dx.doi.org/10.21082/jbio.v7n1.2011.p63-68

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