Phylogenetic and Maturity Analyses of Sixty Soybean Genotypes Used for DNA Marker Development of Early Maturity Quantitative Trait Loci in Soybean

I Made Tasma, Dani Satyawan, Ahmad Warsun, Muhamad Yunus, Budi Santosa

Abstract


Phylogenetic and Maturity Analyses of Sixty Soybean
Genotypes Used for DNA Marker Development of Early
Maturity Quantitative Trait Loci in Soybean. I Made
Tasma, Dani Satyawan, Ahmad Warsun, Muhamad
Yunus, and Budi Santosa. The Indonesian soybean
productivity is still very low with the national average of 1.3
t/ha. One means to improve national soybean productivity is
by manipulating harvest index by cultivating very early
maturing soybean cultivars. Development of early maturing
soybean cultivars can be expedited by using marker-aided
selection. The objective of this study was to select parental
lines having contrasted maturity traits and selected parents
must be genetically distance. The parents then were used to
develop F2 populations for detecting early maturity QTL in
soybean. Maturity tests of 60 soybean genotypes were
conducted at two locations, Cikeumeuh (Bogor) and Pacet
(Cianjur) using a randomized block design with three
replications. Genomic DNA of the 60 genotypes were
analyzed using 18 SSR markers and genetic relationship was
constructed using the Unweighted Pair-Group Method
Arithmatic through Numerical Taxonomy and Multivariate
System program version 2.1-pc. Results showed that the 60
genotypes demonstrated normal distribution in both
locations for days to R1 (32-48d), days to R3 (35-55d), days to
R7 (75-92d), and days to R8 (78-99d). Four early maturing
genotypes and three late genotypes were obtained. Total
SSR alleles observed were 237 with average allele per locus
of 12.6 (3-29), and average PIC value of 0.78 (0.55-0.89).
Genetic similarity among genotypes ranges from 74.8-95%.
At similarity level 77% divided the genotypes into six clusters
(the four selected early maturing genotypes located in
clusters III and IV, while the three late genotypes located in
cluster II). Based on maturity data, pubescent color, and
phygenetic analysis seven parents were selected (four early
maturing genotypes B1430, B2973, B3611, B4433 and three
late genotypes B1635, B1658, and B3570). Twelve F2
populations were developed with the aid of SSR markers
Satt300 dan Satt516. Two of the populations will be used to
develop DNA markers for earliness in soybean.


Keywords


Molecular marker; maturity; genetic distance; soybean; QTL

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DOI: http://dx.doi.org/10.21082/jbio.v7n1.2011.p37-46

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P-ISSN : 1907-1094
E-ISSN : 2549-1547


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