Efikasi Gen RB pada Tanaman Kentang Transgenik Katahdin SP904 dan SP951 terhadap Empat Isolat Phytophthora infestans dari Jawa Barat

Alberta Dinar Ambarwati, S M Sumaraw, Agus Purwito, Muhammad Herman, E. Suryaningsih, Hajrial Aswidinnoor

Abstract


Efficacy of RB gene in transgenic potato Katahdin SP904
and SP951 to West Java isolates of Phytophthora
infestans. A. Dinar Ambarwati, S.M. Sumaraw, Agus
Purwito, M. Herman, E. Suryaningsih, and Hajrial
Aswidinnoor. Potato late blight, caused by Phytophthora
infestans is one of the most devastating plant disease. Potato
yield losses due to this disease ranged from 47-100%. A
major late blight resistance gene, called RB, previously was
identified in the wild potato species Solanum
bulbocastanum. RB gene has been integrated into cultivated
potato Katahdin using Agrobacterium-mediated transformation,
and showed durable and broad spectrum resistance
either in laboratory assay or in confined field trial. Evaluation
of transgenic Katahdin SP904 and SP951 was conducted to
verify whether the RB gene with broad spectrum to all
known races of P. infestans in the United States and in
Toluca, Mexico was also effective against P. infestans
isolates in Indonesia. Efficacy of RB gene was evaluated for
foliar and tuber resistance to West Java isolates. Transgenic
Katahdin were more resistant in foliar than non transgenic
plants, at 14 days after inoculation. Diseases intensity of
transgenic Katahdin SP904 and SP951 were 19.8-43.8%,
whereas non transgenic Katahdin, Granola, and Atlantic
were 46.9-100%. In contrast to the foliar resistance
phenotype, RB-containing tubers in transgenic Katahdin did
not exhibit increased resistance to Lembang, Pangalengan
and Galunggung isolates. Tubers of transgenic Katahdin
SP904, SP951, and non transgenic Katahdin showed lesion
volume of 0.93, 0.91, and 0.91 cm3, respectively. RB gene in
transgenic Katahdin showed efficacy against late blight P.
infestans in foliar, but did not showed efficacy in tuber.
Transgenic Katahdin RB thus providing a potential source of
resistance for breeding programs.


Keywords


RB gene; transgenic potato; Phytophthora infestans; West Java

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DOI: http://dx.doi.org/10.21082/jbio.v7n1.2011.p28-36

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P-ISSN : 1907-1094
E-ISSN : 2549-1547


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