Faktor Virulensi AvrBs3/PthA pada Ras III, Ras IV, Ras VIII, dan IXO93-068 Patogen Hawar Daun Bakteri (Xanthomonas oryzae pv. oryzae)

Dwinita W Utami, Triny S Kadir, Siti Yuriyah

Abstract


AvrBs3/PthA Virulence Factor of Bacterial Leaf Blight
Race III, Race IV, Race VIII, and IXO93-068. Dwinita W.
Utami, Triny S. Kadir, and Siti Yuriyah. Bacterial leaf
blight (BLB) is an important disease of rice and present
throughout many of the rice-growing regions in the world,
also in Indonesia. Xanthomonas oryzae pv. oryzae (Xoo) is
the causal agent and a member of the Protebacteria and like
many other this phyllum have a type III secretion system for
protein virulence effector (PVE) released on their pathogenicity
system. Commonly, PVE in Xanthomonas sp., is
coded by AvrBs3/PthA family gene. This research was
coducted to identify the virulence factor of AvrBs3/PthA on
dominant Indonesian BLB isolates (Race III, Race IV, Ras
VIII, and IXO93-068). This objective was obtained by
sequence analysis through designed markers for members
of the virulence factor AvrBs3/PthA gene family (PthXo4,
avrXa7#38, PthXoS and avrXa7sacB50). Results gave information
that RaceIII is a dependent elicitor race due to no
PVE transcript formed and intraceluler protein target with
RLL type on NLS (nuclear localization signal). RaceIV and
RaceVIII are the virulent race which PVE active formed with
intraceluler protein target and have the RLL and RLLP type
for the NLS signal. While isolate IXO93-068 is a virulen
isolate that active formed a PVE but the extraceluler protein
target is due to no type of NLS. Based on cluster analysis,
Race VIII has a genetic distance closely to PthXoS and
avrXa7sacB50.


Keywords


Bacterial leaf blight; Xanthomonas oryzae pv. oryzae; AvrBs3/PthA virulence factor

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DOI: http://dx.doi.org/10.21082/jbio.v7n1.2011.p1-8

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