Konservasi In Vitro Tanaman Jeruk Besar (Citrus maxima (Burm.) Merr.) Kultivar Srinyonya Menggunakan Osmotikum dan Retardan

Iswari S Dewi, Gani Jawak, Ika Roostika, Muhammad Sabda, Bambang S Purwoko, Widiati H Adil

Abstract


In Vitro Conservation of Pomelo (Citrus maxima (Burm.)
Merr.) cv Srinyonya Using Osmoticum and Retardant.
Iswari S. Dewi, Gani Jawak, Ika Roostika, M. Sabda,
Bambang S. Purwoko, and Widiati H. Adil. Pomelo is an
underutilized citrus fruit with a potential for commercialization.
Only some cultivars have been conserved ex situ, such
as in home yards or in botanical gardens. Such collections
are vulnerable to biotic and abiotic hazards. The goal of the
experiment was to study the effect of osmoticum (sorbitol)
and retardant (ancymidol) on in vitro growth of pomelo.
Four-leaf in vitro shoots of pomelo cultivar Srinyonya were
used as plant materials. Murashige-Skoog (MS) medium was
used as the basal medium for the culture. The trial was
arranged in a completely randomized design with three
replications. The treatments consisting of MS + sorbitol (0,
20, 40, and 60 g/l) and MS + ancymidol (0, 1, 3, and 5 mg/l).
The results indicated that based on plant height, number of
new leaves, and visual plant architecture, sorbitol treatments
from 20-60 g/l retard the growth of the pomelo plant significantly.
On the other hand, ancymidol did not inhibit the
pomelo growth significantly, but it was a suitable osmoticum
for improvement of in vitro plant vigor, increasing green
color of leaf, and increasing root initiation. Leaf senescence
of in vitro plants cultured on media containing sorbitol 40
and 60 g/l began 20 week after storage. The best medium for
conservation of pomelo cv Srinyonya was MS + 20 g
sorbitol/l.


Keywords


Pomelo; in vitro conservation; retardant; osmoticum

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DOI: http://dx.doi.org/10.21082/jbio.v6n2.2010.p84-90

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