Induksi Kalus serta Regenerasi Tunas dan Akar Cabai melalui Kultur In Vitro

Ifa Manzila, Sri H Hidayat, Ika Mariska, Sriani Sujiprihati

Abstract


Callus Induction and Regeneration of Shoot and Root of
Chill through In Vitro Culture. Ifa Manzila, Sri H.
Hidayat, Ika Mariska, and Sriani Sujiprihati. In vitro
culture is one way for a fast and effective plant propagation.
This method is also useful for preliminary selection of plant
resistance to disease, including the chili. In vitro propagation
method for chili has not been widely reported. A study was
conducted to obtain effective techniques for callus induction
and regeneration into shoots on three red chili cultivars (cv)
Gelora, Sudra, and Chili 109. The study consisted of four
activities, namely the induction of callus formation,
induction of embryogenic callus, callus regeneration into
adventitious shoots, and root induction from the adventitious
shoots. Murashige Skoog (MS) medium + 0.6% agar + 3%
sucrose were used as basal medium, 20 ml/bottle. Young
leaves, hypocotyls and root tips of 21-day-old chili seedlings
were used as sources of explants. Each experiment was
arranged in a completely randomized design with 10
replications, one culture bottle for each treatment. The
callus induction experiments using the explants of young
leaf explants, hypocotyl, and root tips were done separately.
Each treatment consisted of explants from the three chili
cultivars on MS medium containing three composition of
growth regulators (PGR) BAP + NAA, 10 explants/bottle. The
embryogenic callus induction was conducted by growing
the callus in bottles containing a medium that contains three
compositions PGR 2,4-D + thidiazuron 0.5 mg/l. Induction of
shoot formation was done by growing the embryogenic
callus on medium containing three composition of plant
growth regulator BAP + NAA. Induction of root formation
was performed by growing adventitious shoots on ½ MS and
1 MS medium + NAA 0.5 to 1.0 mg/l. The results showed that
young leaves are the best explant source for callus and
shoot formations in chili through tissue culture compared
with the hypocotyl and the tip. Gelora is the most responsive
chili cultivar to callus, shoots, and roots formation of in their
respective medium, compared to Sudra and Chile 109. MS
medium containing BAP 3-7 mg/ml and NAA 1 mg/ml can be
used to induce the growth of callus from young leaf
explants, hypocotyl and seedling root tip chili cv Gelora,
Sudra, and Chile 109, but its growth was very slow and did
not produce embryogenic callus. Embryogenic callus
formation can be induced by both non-embryogenic callus
Hak Cipta © 2010, BB-Biogen
growing the callus on MS medium containing 2,4-D 3 mg/l +
thidiazuron 0.5 mg/ l. Formation of callus that can regenerate
into shoots should use an MS medium containing 2,4-D 3
mg/l + thidiazuron 0.5 mg/ l followed by subculture on MS
medium + BAP 3 mg/l + thidiazuron 0.5 mg/l to induce
shoot elongation. Medium ½ MS and 1 MS containing NAA
0.5-1.0 mg/l can be used to induce root formations on shoot
culture of chili cv Gelora but not for cv Chili 109.


Keywords


Callus induction; regeneration; chili; in vitro culture

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DOI: http://dx.doi.org/10.21082/jbio.v6n2.2010.p65-74

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P-ISSN : 1907-1094
E-ISSN : 2549-1547


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