Induksi Kalus dan Regenerasi Beberapa Genotipe Gandum (Triticum aestivum L.) secara In Vitro

Atmitri Sisharmini, Aniversari Apriana, Sustiprijatno Sustiprijatno

Abstract


Callus Induction and In Vitro Plant Regeneration of
Wheat Genotypes (Triticum aestivum L.). Atmitri
Sisharmini, Aniversari Apriana, and Sustiprijatno. Development
of a reliable in vitro plant regeneration procedure for
wheat is a prerequisite for its improvement by genetic transformation.
The purpose of this study was to obtain methods
of callus induction and regeneration of wheat genotypes.
This experiment was conducted at ICABIOGRAD. Immature
embryos from four wheat genotypes, ie Perdix, Naxos Wew,
Combi and Fasan were used to induce callus formation and
regeneration rate of callus. For the preparation of callus
induction medium, MS-L7 basal medium was supplemented
with combination of growth regulators 2,4 dichlorophenoxy
acetic acid (2,4-D) and 4-amino-3,5,6-trichloropicolinic acid
(picloram). While, plant regeneration medium was prepared
using MS basal medium supplemented with combination of
three growth regulators i.e. IAA, BAP and kinetin. The results
showed that genotype, in vitro culture medium and growth
regulators played a dominant role in callus induction and
plantlet regeneration. All the 4 genotypes responded positively
to callus induction, however, variability was observed
not only among the genotypes but also within callus
induction medium used. The best induction medium was
the MS-L7 basal medium supplemented with combination of
phytohormon 4 mg/l 2,4-D + 2 mg/l picloram (GIK-3) which
showed 100% callus induction frequency. Whereas, the best
regeneration medium was shown by MS basal medium with
combination of phytohormon 1.5 mg/l BAP dan 0.5 mg/l
kinetin (RG3). Regarding plant regeneration, Perdix was the
most responsive genotype to be regenerated with regeneration
frequency of 57.33%. The successfully acclimatized
planlets in greenhouse were obtained from Perdix and
Naxos Wew genotypes. These results will potentially facilitate
genetic transformation research of wheat in Indonesia.


Keywords


Wheat (Triticum aestivum L.); callus induction; plant regeneration; growth regulators

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DOI: http://dx.doi.org/10.21082/jbio.v6n2.2010.p57-62

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P-ISSN : 1907-1094
E-ISSN : 2549-1547


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