Pengaruh Iradiasi Sinar Gamma pada Pertumbuhan Kalus dan Keragaman Planlet Tanaman Nilam

Abdul Kadir, Surjono H Sutjahjo, Gustav A Wattimena, Ika Mariska

Abstract


The Effect of Gamma Irradiation on Calli Growth and
Patchouly Planlet Variation. Abdul Kadir, Surjono H.
Sutjahjo, Gustav A. Wattimena, and Ika Mariska. This
research was objected to study the effect of gamma irradiation
on growth of calli and plantlet and phenotypic variation
of patchouly plantlet. Research was conducted at the Tissue
Culture Laboratory of Center for Agricultural Biotechnology
and Genetic Resources Research and Development, Bogor.
Gamma irradiation treatment was done at the Centre for Research
and Development of Isotop and Radiation Technology,
BATAN, Jakarta. The treatment consisted of 5 level of
irradiation i.e. 0 (control), 5, 10, 15, and 20 Gy of gamma irradiation.
The result showed that gamma irradiation of 20 Gy
decrease calli quality index and increased percentage of
calli death and inhibited calli growth at 30, 60 and 90 days
after irradiation, also decrese number of shoots. Gamma irradiation
of 5 Gy and 10 Gy increased growth planlet
compared 15 Gy and 20 Gy, meanwhile gamma irradiation at
20 Gy induced high frequency of phenotypic variation of
patchouly plantlet.


Keywords


Gamma irradiation; calli of patchouli; phenotypic variation

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DOI: http://dx.doi.org/10.21082/jbio.v3n1.2007.p24-31

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P-ISSN : 1907-1094
E-ISSN : 2549-1547


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