Isolasi dan Karakterisasi Aktinomisetes Penghasil Antibakteri Enteropatogen Escherichia coli K1.1, Pseudomonas pseudomallei 02 05, dan Listeria monocytogenes 5407

Dwi Ningsih Susilowati, Ratih Dewi Hastuti, Erny Yuniarti

Abstract


Isolation and Characterization of Actinomycetes Producing
Antibacterial Compound into Enteropatogenik
Escherichia coli K1.1, Pseudomonas pseudomallei 02 05
and Listeria monocytogenes 5407. Dwi N. Susilowati,
Ratih D. Hastuti, and Erny Yuniarti. The resistance of
bacterial pathogens to some antibacterial agents and side
effects of the antibacterial usage demanded discovery of
new effective, safe, and active antibacterial compounds.
Some pathogenic bacteria, such as enteropathogen Escherichia
coli (EPEC) that cause diarrhoea on children and
infants, Pseudomonas pseudomallei that cause melioidosis
on human and animal, and Listeria monocytogenes that
cause listeriosis on newly born babies mortality and death of
pregnant woman. Actinomycetes is the largest bacterial
group that produce antibiotics. More than 10,000 antibacterial
compounds had been discovered, two-third of
them were produced by this bacterial group. A study was
done to isolate and characterize Actinomycetes producing
antibacterial compounds effective against EPEC K1.1 and P.
pseudomallei 02 05. Soil samples were taken from 39
locations in Indonesia and 115 actinomycetes isolates were
obtained. Two of the isolates, i.e., isolate A3.5 that was
effective against P. pseudomallei 02 05 and isolate F6.1 that
was effective against EPEC K1.1 evaluated further. The
isolate A3.5 had an optimum time 72 hours to produce antibacterial
compound, while F6.1 took 96 hours. The antibacterial
compounds produced by both isolates were dissolve
in the a 70% ethyl acetate solution, but not in a 40oC
warm methanol solution because it is very dissolved. The
antibacterial compound extracted from the isolate A3.5 had
a similar effectiveness to antibiotics bacithracyn 10 unit and
neomycin 30 g. On the other hand, the antibacterial
compound extracted from isolate F6.1 had a similar effectiveness
to antibiotics colistin 10 g and doxyciclin 30 g.
Further identification of the isolates suggested that both of
them belongs to the genera Streptomyces.


Keywords


Actinomycetes; antibacterial compound; Escherichia coli K1.1; Pseudomonas pseudomallei 02 05; Listeria monocytogenes 5407

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DOI: http://dx.doi.org/10.21082/jbio.v3n1.2007.p15-23

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