Spektrum Ketahanan Galur Haploid Ganda Turunan IR64 dan Oryza rufipogon yang mengandung QTL Ketahanan terhadap Penyakit Blas (Pir)

Dwinita Wikan Utami, Alberta Dinar Ambarwati, Aniversari Apriana, Atmitri Sisharmini, Ida Hanarida, Didier Tharreau, Santosa Santosa

Abstract


Resistance Spectrum of Double Haploid Lines Derived
from IR64 and Wild Rice Species, Oryza rufipogon
Contained the Blast Resistance QTL (Pir). Dwinita W.
Utami, A. Dinar Ambarwati, Aniversari Apriana, Atmitri
Sisharmini, Ida Hanarida, Didier Tharreau, and Santosa.
This study was initiated to determine the spectrum resistance
of the candidate durable blast resistance variety contained
the QTL (quantitative trait locus), Pir1 and 2. This QTL
was mapped on chromosome 2 detected using the advanced
backcross population (BC5) from the wild rice species
Oryza rufipogon to IR64. Pir (1 and 2) also established
on double haploid (DH) population derived from the selected
lines of BC2F3 population, progenies from the same
parents. The DH lines were developed to speed up the fixation
process of the recessive alleles in the selected lines.
Near isogenic lines with different blast resistance genes and
combination were used in this study comparing to the DH
population on their resistance spectrum using the known
avr gene isolates both on green house and field screening.
The determination of the resistance spectrum will useful on
the prediction of durability of blast resistance gene in DH
population. The results of spectrum resistance test in green
house and field showed that Pir1and Pir2 segregated on 1 : 1
proportion related with specific respond to blast avr gene
PH14 and CM28 resistance. Pir1 was identic to Pi33 or Pi25
and Pir2 to Pitq5 on spectrum resistance.


Keywords


Double haploid lines; IR64; Oryza rufipogon

Full Text:

PDF


DOI: http://dx.doi.org/10.21082/jbio.v3n1.2007.p1-8

Refbacks

  • There are currently no refbacks.




Copyright (c) 2016 Jurnal AgroBiogen

 Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.


           


P-ISSN : 1907-1094
E-ISSN : 2549-1547


Jurnal AgroBiogen

Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Jl. Tentara Pelajar 3A, Bogor 16111
Jawa Barat, Indonesia
Telp.: (0251) 8339793, 8337975
Faks.: (0251) 8338820
E-mail: jurnal.agrobiogen@gmail.com
Situs: http://biogen.litbang.pertanian.go.id



View My Stats