Imron Riyadi, Sumaryono Sumaryono


The application of gamma irradiation on plant materials may increase the genetic variation of the offspring with useful traits. The experiment was conducted to determine the effect of irradiation dosage of gamma ray on growth and development of sago palm (Metroxylon sagu) calli. Friable calli of sago palm derived from suspension culture were used as a material source. The primary calli were initiated from apical meristematic tissues of sago palm suckers of Alitir variety from Merauke, Papua. The treatments used were dosage of gamma ray irradiation at 0, 5, 10, 15, 20 and 25 Gy. The treated calli were then subcultured on modified Murashige and Skoog (MMS) solid medium containing 3% sucrose and 0.1% activated charcoal and added with 1 mg l-1 2,4-D and 0.1 mg l-1 kinetin. The results showed that at all irradiation dosages, calli biomass increased significantly. The highest proliferation of calli biomass of 5.33 folds from the initial culture after 4 weeks was achieved at gamma irradiation of 25 Gy, whereas the lowest proliferation of calli biomass of 3.4 folds was achieved at control. The best development of embryogenic calli was obtained at 10 Gy that produced 100% somatic embryos, whereas the lowest somatic embryo formation at 0% was obtained at 0 and 25 Gy after one subculture. High response of somatic embryo induction to gamma irradiation at 10 Gy may increase production of somatic embryos. These results can be used in in vitro breeding of sago palm via mutagenesis to create new elite varieties.


Metroxylon sagu; gamma irradiation; embryogenic calli; somatic embryo

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Agisimanto, D., M.N. Noor, R. Ibrahim and A. Mohamad. 2016. Gamma irradiation effect on embryogenic callus growth of Citrus reticulata cv. Limau Madu. Sains Malaysiana 45(3): 329–337.

Bao, J.S., Z.H. Ao and J.L. Jane. 2005. Characterization of physical properties of flour and starch obtained from gamma-irradiated white rice. Starch 57: 480–487.

Barakat, M.N. and H. El-Sammak. 2011. In vitro mutagenesis, plant regeneration and characterization of mutants via RAPD analysis in Baby’s breath Gypsophila paniculata L. Australian J. Crop Sci. 5(2): 214–222.

Bintoro, M.H., M. Mashud and H. Novarianto. 2007. Status teknologi sagu. hlm. 76–94. Dalam E. Karmawati et al. (Ed.). Prosiding Lokakarya Pengembangan Sagu di Indonesia, Batam.

Ehara, H. 2009. Potency of sago palm as carbohydrate resource for strengthening food security program. J. Agron. Indonesia 37(3): 209–219.

Flach, M. 1997. Sago Palm Metroxylon sagu Rottb. Promoting the Conservation and Use of Underutilized and Neglected Crops. 13. International Plant Genetic Resources Institute, Rome. 76 pp.

IAEA. 2012. Plant Mutation Breeding and Biotechnology. International Atomic Energy Agency. Gutenberg Press Ltd. 608 pp.

Kanro, M.Z., A. Rouw, A. Widjono, Syamsuddin, Amisnaipa, dan Atekan. 2003. Tanaman sagu dan pemanfaatannya di Propinsi Papua. Jurnal Penelitian dan Pengembangan Pertanian 22(3): 116–124.

Konan, E.K., J.Y. Kouadio, A. Flori, T.D. Gasselin and A. Rival. 2007. Evidence for an interaction effect during in vitro rooting of oil palm (Elaeis guineensis Jacq.) somatic embryo-derived plantlets. In Vitro Cell. Dev. Biol. Plant 43: 456–466.

Muruganantham, M., S. Amutha and A. Ganapathi. 2010. Somatic embryo productions by liquid shake culture of embryogenic calluses in Vigna mungo (L.) Hepper. In Vitro Cell. Dev. Biol. Plant. 46: 34–40.

Novero, A., A.G. Delima, J. Acaso and L.M. Baltores. 2010. The influence of osmotic concentration of media on the growth of sago palm (Metroxylon sagu Rottb.) in vitro. Australian J. Crop Sci. 4(6): 453–456.

Piri, I., M. Babayan, A. Tavassoli and M. Javaheri. 2011. The use of gamma irradiation in agriculture. Afr. J. Microbiol. Res. 5(32): 5806–5811.

Riyadi, I., J.S. Tahardi and Sumaryono. 2005. The development of somatic embryos of sago palm (Metroxylon sagu Rottb.) on solid media. Menara Perkebunan 69(2): 46–57.

Rohani, O., R. Samsul Kamal, S. Rajinder and B. Mohd-Nazir. 2012. Mutation induction using gamma irradiation on oil palm (Elaeis guineensis Jacq.) cultures. J. Oil Palm Res. 24: 1448–1458.

Rostiwati, T., Y. Lisnawati, S. Bustomi, B. Leksono, D. Wahyono, S. Pradjadinata, R. Bogidarmanti, D. Djaenudin, E. Sumadiwangsa dan N. Haska. 2008. Sagu (Metroxylon spp.) sebagai sumber energi bioetanol potensial. Pusat Penelitian dan Pengembangan Hutan Tanaman, Bogor. 75 hlm.

Singhal, R.S., J.F. Kennedy, S.M. Gopalakrishnan, A. Kaczmarek, C.J. Knill and P.F. Akmar. 2008. Industrial production, processing, and utilization of sago palm-derived products. Carbohydrate Polymers 72: 1–20.

Sumaryono, I. Riyadi and P.D. Kasi. 2009. Clonal propagation of sago palm (Metroxylon sagu Rottb.) through tissue culture. J. Appl. Industrial Biotechnol. Trop. Region. 2(1): 1-4.

Sumaryono, W. Muslihatin and D. Ratnadewi. 2012. Effect of carbohydrate source on growth and performance of in vitro sago palm (Metroxylon sagu Rottb.) plantlets. Hayati J. Biosci. 19(2): 88–92.

Sung, N.Y., E.B. Byun, D.S. Song, Y.B. Jin, J.K. Kim, J.H. Park, B.S. Song, P.M. Jung, M.W. Byun, J.W. Lee, S.H. Park and J.H. Kim. 2013. Effect of gamma irradiation on mistletoe (Viscum album) lectin-mediated toxicity and immunomodulatory activity. FEBS Open Biol. 3: 106–111.

Tahardi, J.S., N.F. Sianipar and I. Riyadi. 2002. Somatic embryogenesis in sago palm (Metroxylon sagu Rottb.). pp. 75–81. In K. Kaimuna et al. (Eds.) New Frontiers of Sago Palm Studies. Universal Academic Press, Inc., Tokyo.

Tarigans, D.D. 2001. Sagu memantapkan swasembada pangan. Warta Penelitian dan Pengembangan Pertanian 23(5): 1–3.

Yasmin, S., I.A. Khan, A. Khatri, N. Seema, M.A. Siddiqui and S. Bibi. 2011. Plant regeneration from irradiated embryogenic callus of sugarcane. Pak. J. Bot. 43(5): 2423–2426.

DOI: http://dx.doi.org/10.21082/ijas.v17n1.2016.p35-40


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