Riry Prihatini, Norihan Mohamad Saleh


Natural exposure of extremely low frequency electromagnetic field (ELF-EMF) occurs in the environment and acts as one of the abiotic factors that affect the growth and development of organisms. This study was conducted to determine the effect of ELF-EMF on the tissue cultured banana and slipper orchid chlorophyll content as one of the indicators in measuring plant photosynthetic capacity. Four days old banana (Musa sp. cv. Berangan) corm and seven days old slipper orchid (Paphiopedilum rothschildianum) cultures were exposed to 6 and 12 mT ELF-EMF generated by controllable ELF-EMF built up machine for 0.5, 1, 2 and 4 hours. After exposure, the banana and orchid cultures were incubated at 25° C for 8 and 16 weeks, respectively. The results showed that the ELF-EMF exposure had different effects on banana and slipper orchid cultures though both plant species belong to monocotyledon. The highest increase in chlorophyll content on banana was resulted by the high intensity and long duration of ELF-EMF exposure (12 mT for 4 hours), whereas on slipper orchid the modest and short duration of ELF-EMF exposure produced the most excessive chlorophyll content. Different ELF-EMF exposures (12 mT for 4 hours and 6 mT for 30 minutes) had potential to be applied on each plant to improve in vitro plant (banana and slipper orchid, respectively) growth. The increased chlorophyll and carotene/xanthophyll content on banana indicated that the banana was more tolerant to ELF-EMF exposure compared to slipper orchid. 


Banana; orchid; carotene; chlorophyll; electro-magnetic field

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Apasheva, L.M., A.V. Lobanov and G.G. Komissarov. 2006. Effect of alternating electromagnetic field on early stages of plant development. Doklady Biochem. Biophys. 406: 1–3.

Apel, K. and H. Hirt. 2004. Reactive oxygen species: Metabolism, oxidative stress, and signal transduction. Ann. Rev. Plant Biol. 55: 373–399.

Aremu, A.O., M.W. Bairua, L. Szucova, J.F. Finniea and J. van Staden. 2012. The role of meta-topolins on the photosynthetic pigment profiles and foliar structures of micropropagated ‘Williams’ bananas. J. Plant Physiol. 169: 1530–1541.

Damaraju, S., S. Schlede, U. Eckhardt, H. Lokstein and B. Grimm. 2011. Functions of the water soluble chlorophyll-binding protein in plants. J. Plant. Physiol. 168: 1444–145.

Frija, G., J. Bittoun, G.P. Krestin and D. Norris. 2006. European directive on electromagnetic field. Eur. Rad. 16: 2886–2889.

Furse, C., D.A. Christensen and C.H. Durney. 2008. Basic introduction to bioelectromagnetics. CRC Press, Boca Raton. 356 pp.

Gao, S., J. Gao, X Zhu, Y. Song, Z. Li, G. Ren, X. Zhou and B. Kuai. 2016. ABF2, ABF3, and ABF4 promote ABA-mediated chlorophyll degradation and leaf senescence by transcriptional activation of chlorophyll catabolic genes and senescence-associated genes in Arabidopsis. Molecular Plant 9 (9): 1272–1285.

Gitelson, A.A., Y. Gritz and M.N. Merzlyak. 2003. Relationships between leaf chlorophyll content and spectral reflectance and algorithms for non-destructive chlorophyll assessment in higher plant leaves. J. Plant Physiol. 160: 271–282.

Grandolfo, M. 2009. Worldwide standards on exposure to electromagnetic fields: An overview. Environmentalist 29:109–117.

Guan, J.Z., S.B. Zhang, K.Y. Guan, S.Y. Li and H. Hu. 2010. Leaf anatomical structure of Paphiopedilum and Cypripedium and their adaptive significance. J. Plant Res. 124: 289–298.

Hanninen, H., P. Huttunen and R. Ekman. 2011. Electromagnetic radiation exposure and its bioindication – An overview. J. Env. Sci. 23: 1409–1414.

Hotensteiner, S. 2006. Chlorophyll degradation during senescence. Annu. Rev. Plant. Biol. 57: 55–57.

Hortensteiner, S. and B. Krautler. 2011. Chlorophyll breakdown in higher plant. Biochem. Biophys. Acta. 8: 977–988.

Huang, H.H. and S.R. Wang. 2007. The effects of 60 Hz magnetic fields on plant growth. Nat. Sci. 5: 59–68.

Kadlecek, P., B. Rank and I. Ticha. 2003. Photosynthesis and photoprotection in Nicotiana tabacum L. in vitro grown plantlets. J. Plant. Physiol. 160. 1017–1024.

Kato, M. and S. Shimizu. 1987. Chlorophyll metabolism in higher plants. VII. Chlorophyll degradation in senescing tobacco leaves; phenolic-dependent peroxidative degradation. Can. J. Bot. 65(4): 729–735.

Kostoff, R.N. and C.G.Y. Lau. 2013. Combined biological and health effects of electromagnetic field and other agents in the published literature. Technological Forecasting and Social Change 80: 1331–1349.

Lambrozo, J. and M. Souques. 2012. Electricity and extremely low frequency electric and magnetic fields. In A. Perrin and M. Souques (Eds.). pp. 35–50. Electromagnetic Fields, Environment and Health. Springer-Verlag, France.

Li, Z.Y., S.Y. Guo, L. Li and M.Y. Cai. 2007. Effect of electromagnetic field on the batch cultivation nutritional composition of Spirulina platensis in air-lift photobioreactor. Bioresour. Technol. 98: 700–705.

Liu, Z., R. Cheng, W. Xiao, Q. Guo, Y. Wang, N. Wang and Y. Wang. 2015. Leaf gas exchange, chlorophyll fluorescence, non-structural carbohydrate content and growth responses of Distylium chinense during complete submergence and subaerial re-emergence. Aquatic Bot. 124: 70–77.

Mafakheri, A., A. Siosemardeh, B. Bahramnejad, P.C. Struik and Y. Sohrabi. 2010. Effect of drought stress on yield, proline and chlorophyll contents in three chickpea cultivars. Aus. J. Crop. Sci. 4: 580–584.

Mahmood, M., B.B. Ooi, T. Mahmud and S. Subramaniam. 2011. The growth and biochemical responses on in vitro cultures of Oncidium taka orchid to electromagnetic field. Aus. J. Crop. Sci. 5: 1577–1587.

Mrozynski, G. and M. Stallein. 2013. Electromagnetic Field Theory. Vieweg+Teubner Verlag, Weosbaden.

Muas, I. 2010. Petunjuk teknis: Teknologi budidaya pisang sehat. Balai Penelitian Tanaman Buah Tropika, Solok. iv + 59 hlm.

Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays for tobacco tissue cultures. Physiol. Plant. 15: 473–497.

Ni, Z., E.D. Kim, M. Ha, E. Lackey, J. Liu, Y. Zhang, Q. Sun and Z.J. Chen. 2009. Altered circadian rhythms regulate growth vigor in hybrids and allopolyploids. Nature 457: 327–332.

Nyachiro, J.M., K.G. Briggs, J. Hoddinott and A. Johnson-Flanagan. 2001. Chlorophyll content of spring wheat flag leaves grown under elevated CO2 and other environmental stresses within the ESCAPE-project. Eur. J. Agron. 10: 197–203.

Pareks, D., K.R.M. Purani and H.S. Srivastava. 1990. Inhibition of chlorophyll biosynthesis by cadmium in greening maize leaf segments. Biochemie Physiologie der Pflanzen 186: 239–242.

Pazur, A. and V. Rassadina. 2009. Transient effect of weak electromagnetic fields on calcium ion concentration in Arabidopsis thaliana. BMC Plant. Biol. 9: 47. Retrieved from [April 4th 2010]. DOI: 10.1186/1471-2229-9-47.

Piacentini, M.P., D. Fraternale, E. Piatti, D. Ricci, F. Vetrano, M. Dacha and A. Accorsi. 2001. Senescence delay and change of antioxidant enzyme levels in Cucumis sativus L. etiolated seedlings by ELF magnetic fields. Plant Sci. 161: 45–53.

Pietruszewski, P., S. Muszynski and A. Dziwulska. 2007. Electromagnetic fields and electromagnetic radiation as non-invasive external simulations for seeds (selected methods and responses). Intl. Agrophy. 21: 95–100.

Racuciu, M. and D.E Creanga. 2005. Biological effect of low frequency electromagnetic field in Curcumis pepo. Proceedings of the Third Moscow International Symposium on Magnetis. pp. 278–282.

Racuciu, M., S. Miclaus and D.E. Creana. 2009. The response of plant tissue to magnetic fluid and electromagnetic exposure. Romanian J. Biophys. 19(1): 73–82.

Ravazzani, P. 2008. The interpretation of the results of the research on electromagnetic fields and health in Europe: the EC Coordination Action EMF-NET. Annual Telecommunication 63: 11–15.

Sandu, D.D., C. Goeceanu, A. Ispas, I. Creange, S. Miclaus and D.E. Creange. 2005. A preliminary study of ultra high electromagnetic fields effect on black locust chlorophylls. Acta Biologica Hungarica 56: 2–11.

Suzuki, W., W. Sugawara, K. Miwa and M. Morikawa. 2014. Plant growth-promoting bacterium Acinetobacter calcoaceticus P23 increases the chlorophyll content of the monocot Lemna minor (duckweed) and the dicot Lactuca sativa (lettuce). J. Biosci. Bioeng. 118: 41–44.

Waite, G.N., S.J.P. Egot-Lemaire and W.X. Balcavage. 2011. A novel view of biologically active electromagnetic field. Environmentalist 31: 107–113.

Wu, H., W. Jiang, C. Liu and Y. Deng. 2015. Growth, pigment composition, chlorophyll fluorescence and antioxidant defences in the red alga Gracilaria lemaneiformis (Gracilariales, Rhodophyta) under light stress. South Afr. J. Bot. 100: 27–32.



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