EVALUASI EFIKASI FORMULA Pseudomonas fluorescens UNTUK PENGENDALIAN PENYAKIT LAYU BAKTERI (Ralstonia solanacearum) NILAM

NFN Nasrun, NFN Burhanuddin

Abstract


Penyakit layu bakteri (Ralstonia solanacearum) merupakan masalah utama tanaman nilam. Pengendalian hayati dengan memanfaatkan Pseudomonas fluorescens untuk mengendalikan penyakit layu bakteri nilam perlu dikembangkan. Tujuan penelitian ini adalah mengetahui pengaruh masa penyimpanan formula dengan bahan aktif kombinasi P. fluorescens Pf19 dan Pf101 dan efektifitasnya untuk mengendalikan penyakit layu bakteri dan meningkatkan pertumbuhan nilam. Penelitian dilaksanakan di kebun petani di Pasaman Barat Sumatera Barat sejak Maret 2010 sampai Februari 2011. Perlakuan disusun dalam rancangan acak kelompok (RAK) faktorial dengan dua faktor dan 3 ulangan. Faktor pertama adalah lama penyimpanan formula yaitu 0, 30, 60, 90, 120 dan150 hari, dan faktor kedua adalah tingkat umur tanaman yaitu benih, bibit dan tanaman dewasa. Hasil penelitian menunjukkan formula kombinasi P. fluorescens Pf19 dan Pf101 mampu menurunkan perkembangan penyakit layu bakteri dan meningkatkan pertumbuhan dan produksi tanaman. Formula kombinasi P. fluorescens Pf19 dan Pf101 yang disimpan selama 30 sampai 120 hari yang diaplikasi pada benih dan bibit tanaman nilam efektif dalam mereduksi penyakit layu bakteri dengan masa inkubasi gejala penyakit 134,10-175,00 hari setelah tanam dengan intensitas penyakit           11,31-24,15%, serta meningkatkan produksi daun nilam dengan berat basah 45,97-55,86 g petak-1 dan berat kering daun 18,82-25,60 g petak-1.


Keywords


Formulasi; fase umur tanaman; masa penyimpanan formula; Pseudomonas fluorescens; penyakit layu bakteri

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References


Abdul Wahab NI, R Nulit, IA Seman and H Omar. 2014. Capability of Powder Formulation of Bioorganic Containing Pseudomonas Gano EB3 for Promoting the Growth of Oil Palm Seedlings. International Journal of Agriculture and Crop Sciences 7(12): 988-992.

Anitha A and M Rabeeth. 2009. Control of Fusarium Wilt of Tomato by Bioformulation of Streptomyces griseus in Green House Condition African Journal of Basic & Applied Sciences 1(1-2): 9-14.

Ardebili ZO, NO Ardebili and SMM Hamdi. 2011. Physiological Effects of Pseudomonas fluorescens CHAO on Tomato (Lycopersicon esculentum Miel) plants and its possible impact on Fusarium oxysforum f.sp. Lycopersici. Australian Journal of Crop Science 5(12): 1631-1638.

Ardakani SS, A Heydari, N Khorasani and R Arjmandi. 2010. Development of New Bioformulations of Pseudomonas fluorescens and Evaluation of these Products against Damping-off of Cotton Seedlings. Journal of Plant Pathology 91(1): 83-88.

Campbell R. 1989. Biological Control of Microbial Plant Pathogens, Cambridge University Press, Cambridge, 218 p.

Chakravarty G and MC Kalita. 2011. Comparative evaluation of Organic formulations of Pseudomonas fluorescens Based Biopesticides and Their Application in the Management of Bacterial Wilt of Brinjal (Solanum melongena L.). African Journal of Biotechnology 10(37): 7174-7182.

Chakravarty G and MC Kalita. 2012. Biocontrol Potential of Pseudomonas fluorescens Against Bacterial Wilt of Brinjal and Its Possible Plant Growth Promoting Effects. Annals of Biological Research 3(11): 5083-5094.

Chrisnawati, Nasrun dan T Arwiyanto. 2009. Pengendalian Hayati Penyakit Layu Bakteri Nilam Menggunakan Bacillus spp dan Pseudomonad fluoresen. Jurnal Littri 15(3): 116-123.

Disbun Sumbar [Dinas Perkebunan Sumatera Barat]. 2011. Produktivitas panen nilam sumber stagnan. http://Pmi.or.id/2011/04/22/produktivitas-panen- nilam-sumbar-stagnan/ Tembolok. Dinas Perkebunan TK I Sumatera Barat. (24 Maret 2012).

Ditjenbun [Direktorat Jenderal Perkebunan]. 2011. Nilam. Statistik Perkebunan Indonesia 2011-2012. Direktorat Jenderal Perkebunan, Jakarta. 20 hlm.

Fallahzadeh V, M Ahmadzadeh, A Marefat and K Ghazanfary. 2009. Applcation of Rhizobacteria for Induction of Systemic Resistance to Bacterial Blight of Cotton Caused by Xanthomonas campestris pv. Malvacearum Using Fluorescent Pseudomonads of Rhizosphere. Journal of Plant Protection Research 49(4): 416-420.

Haggag WM and Abo El Soud. 2012. Production and Optimization of Pseudomonas fluorescens Biomass and Metabolites for Biocontrol of Strawberry Grey Mould. American Journal Plant Sciences 3: 836-845.

Handoyono. 2011 Ekspor Minyak Nilam. http:// industri.kontan.co.id/stok-melimpah-harga-minya k-nilam-terus-m/Tembolok. [21 Maret-2012].

Harni R dan MSD Ibrahim. 2011. Potensi Bakteri Endofit Menginduksi Ketahanan Tanaman Lada terhadap Infeksi Meloidogyne incognita. Jurnal Littri 17(3): 118-123.

Jambhulkar PP and P Sharma. 2014. Development of Bioformulation and Delivery System of Pseudomonas fluorescens Against Bacterial Leaf Blight of Rice (Xanthomonas oryzae pv.oryzae). J. Environmental Biology 35: 843-849.

Jeyatakshmi C, K Madhiazhagan and C Rettinassababady. 2010. Effect of Different Methods of Application of Pseudomonas fluorescens Against Bacterial Leaf Blight Under Direct Sown Rice. Journal of Biopesticides 3(2): 487-488.

Khaeruni A, A Wahab, M Taufik dan GAK Sutariati. 2013. Kefektifan waktu aplikasi formulasi rizobakteri indigenus untuk mengendalikan layu Fusarium dan Meningkatkan Hasil Tanaman Tomat di Tanah Ultisol. J. Hort. 23(4): 365-371.

Khalimi K and DN Suprapta. 2011. Induction of Plant Resistance Against Soybean Stunt Virus Using some Formulations of Pseudomonas aeruginosa. J. ISSAAS 17(1): 98-105.

Khabbaz SE and PA Abbasi. 2014. Isolation, Characterization, and Formulation of Antagonistic Bacteria for the Management of Seedlings Damping-off and Root Rot Disease of Cucumber. Can. J. Microbiology 60: 25-33.

Landa BB HAE de Werd, BB McSpadden Gardener, and DM Weller. 2002. Comparison of Three Methods for Monitoring Populations of Different Genotypes of 2,4-diacethylphloroglucinol-producing Pseudomonas fluorescens in Rhizosphere. Phythopatholgy 92: 129-137.

Manidipa R, SG Dutta and RCh Venkata. 2013. Pseudomonads: Potential Biocontrol Agents of Rice Diseases. Research Journal of Agriculture and Forestry Sciences 1(9): 19-25.

Manikandan R, D Saravanakumar, L Rajendran, T Raguchander and R Samiyappan. 2010. Standardization of Liquid Formulation of Pseudomonas fluorescens PF1 for Its Efficacy Against Fusarium Wilt of Tomato. Biological control 54: 83-89.

Manjunatha H, MK Naik, MB Patil, R Lokesha and SN Vasudevan. 2012. Isolation and Characterization of Native Fluorescent Pseudomonads and Antagonistic Activity Against Major Plant Pathogens. Karnataka. J. Afric. Sci. 25(3): 346-349.

Meena B. 2011. Effect of Pseudomonas fluorescens Pf1 Formulation Application on Rhizosphere and Phyllosphere Population in Groundnut. International Journal of Plant Protection 4(1): 92-94.

Muthukumar A, R Bhaskaran and K Sanjeevkumar. 2010. Efficacy of Endophytic Pseudomonas fluorescens (Trevisan) Migula Against Chilli Damping-off. Journal of Biopesticides 3(1 Special Issue): 105-109.

Nasrun, Christanti, T Arwiyanto dan I Mariska. 2005. Pengendalian Penyakit Layu Bakteri Nilam Menggunakan Pseudomonad fluoresen. Jurnal Littri 11(1): 19-24.

Nasrun, S Christatnti, T Arwiyanto dan I Mariska. 2007a. Karakteristik Fisiologis Ralstonia solanaceraum Penyebab Penyakit Layu Bakteri Nilam. Jurnal Littri 13(2): 43-48.

Nasrun, T Arwiyanto dan I Mariska. 2007b. Pemanfaatan Pseudomonad fluoresen sebagai Agens Pengimbas Ketahanan Tanaman Dalam Mengendalikan Penyakit Layu Bakteri dan Meningkatkan Pertumbuhan Nilam. Status Teknologi Hasil Penelitian Nilam. Kementrian Riset dan Teknologi Tahun 2007. 44 hlm.

Nasrun, T Arwiyanto dan I Mariska. 2008. Pemanfaatan Produk Pseudomonad fluoresen sebagai Agens Pengimbas Ketahanan Tanaman Dalam Mengendalikan Penyakit Layu Bakteri dan Meningkatkan Pertumbuhan Nilam. Status Teknologi Hasil Penelitian Nilam Tahun 2008. Kementrian Riset dan Teknologi. 48 hlm.

Nawangsih AA, R Aditya, B Tjahjono, H Negishi and K Suyama. 2012. Bioefficacy and Characterization of Plant Growth Promoting Bacteria to Control the Bacterial Wilt Disease of Peanut in Indonesia. J. ISSAAS 8(1): 185-192.

Noumavo PA, E Kochani, YO Didagbe, A Adjanohoun, M Allagbe, R Sikirou, EW Gachomo, SO Kotchoni and L Baba-Moussa. 2013. Effect of Different Plant Growth Promoting Rhizobacteria on Maize Seed Germination and Seedling Development. American Journal of Plant Sciences 4: 1013-1021.

Ramyasmruthi S, O Pallavi, S Pallavi, K Tilak and S Srividya. 2012. Chitinolytic and Secondary Metabolite Producing Pseudomonas fluorescens Isolated From Solanaceae Rhizosphere Effective Against Broad Spectrum Fungal Phytopathogens. Asian J. Plant Science and Research 2(1): 16-24.




DOI: http://dx.doi.org/10.21082/bullittro.v27n1.2016.67-76

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