POTENCY OF ANTIOXIDANT, ANTICHOLESTEROL AND PLATELET ANTIAGGREGATION OF BLACK TEA (Camelia sinensis )

Wahyu Widowati, Tati Herlina, Hana Ratnawati, Tjandrawati Mozef, Victor Immanuel

Abstract


Epidemiological studies have shown an in-verse correlation betweendiets rich in po-lyphenols, flavonoids and reduced risk of cardiovasculardisease. These associationswere mainly ascribed to the antioxidant, antiaggregation and anticholesterol capa-city of polyphenols. This association has been explained that atherogenesis is ini-tiated by hypercholesterol, lipid peroxida-tion and hyper aggregation platelet. The research was carried out to evaluate anti-oxidant, anticholesterol and antiaggrega-tion activities of methanol extract and frac-tions of black tea (Camelia sinensis). To evaluate antioxidant activity of methanol extract and fractions were compared  with (-)-Epigallocatechine 3-gallate (EGCG), an-ticholesterol activity were compared with simvastatin and antiaggregation activity were compared with aspirin. Antioxidant activity using the 1,1-diphenyl-2-picryl-hy-drazyl (DPPH) free radical scavenging acti-vity at two concentrations 100 µg/mL, 50 µg/mL and anticholesterol was cholesterol oxidase activity at two concentrations 0.005 µg/mL, 0.0025 µg/mL, the antiag-gregation  activity  used epinephrine (EPN)

agonist at two concentrations 300 μM, 75 μM and one concentration 37.1 µg/mL of methanol extract and fractions of black tea. To know the difference of treatment, the data were analysed using Analysis of Variance (ANOVA) and be continued Dun-can’s new multiple range test. The results showed that DPPH scavenging activity were high (88.59-93.556%), the anticho-lesterol showed high activity (93.663-97.434%) and the antiaggregation sho-wed low up to high activity (79.967-4.31%). Using Duncan’s new multiple ra-nge test showed that anti-oxidant activity were not different among extract and fractions of black tea,the highest anti-cholesterol activity was black tea extract 0.005 µg/mL (97.434%) and the highest antiaggregation activity was ethyl acetate fraction on 75 μM EPN agonist (4.310 %), water fraction 37.1 µg/mL with 300 μM EPN agonist (21.833%).


Keywords


Black tea; Camelia sinensis; an-tioxidant; anticholesterol; anti-aggregation; flavonoids; cardio-vascular disease

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References


Buck, R., J.J. Pastore, F. Giraud, J-C, Sulpice and P.A. Janmey. 2003. Fla- vonoid inhibition of platelet procoagu- lant activity and phosphoinositide synthesis. J. Throm. Haemostasis. 1 : 1820-1828.

Cabrera, C., R. Artacho and R. Gime´nez. 2006. Beneficial effects of green tea- A Review. J. Am. Coll. Nutr. 25 : 79-99.

Cooper, R., J. Morre and D.M. Morre. 2005. Medicinal benefits of green tea: Part I. Review of Noncancer Health Benefits. J Altern. Comple-mentary. Medicine. 11: 521-528.

Duffy, S.J., J.F.Jr. Keaney, M.Holbrook, N. Gocke, P.L. Swerdloff, B. Frei and J.A. Vita. 2001. Short-and long-term black tea consumption reverses en- dothelial dysfunction in patients with coronary artery disease. Circulation. 104 : 151–156.

Frei, B and J.V. Higdon. 2003. Anti- oxidant activity of tea polyphenols in vivo : evidence from animal studies. J. Nutr. 133 : 3275S-3284S.

Frum, Y and A.M. Viljoen. 2006. In vitro 5-lipoxygenase and antioxidant acti- vities of South African medicinal plants commonly used topically for

skin disease. Skin. Pharmacol. Physiol. 19 : 329-335.

Geleijnse, J.M., L.J. Launer, D.A.M. van der Kuip, A. Hofman and J.C.M. Witteman. 2002. Inverse association of tea and flavonoid intakes with incident myocardial infarction: the Rotterdam Study. Am. J. Clin. Nutr.

: 880-886.

Gordon, M.H. 2001. Measuring antioxi- dant activity. In Pokorny, J., N. Yanishlieva, M. Gordon. (eds). Anti- oxidants in food. Boca Raton : Woodhead Publihing Limited. Cam- bridge England.

Graham, H.N. 1992. Green tea compo- sition, consumption, and polyphenol chemistry. Prev. Med. 21 : 334-50.

Grassi, D., A. Aggio, L. Onori, G. Croce, S. Tiberti, C. Ferri, L. Ferri and G. Desideri. 2008. Tea, flavonoids, and nitric oxidemediated vascular reacti- vity. J. Nutr. 138 : 1554S-1560S.

Helena Laboratories. 2008. Helena pla- telet aggregation system. Beau- mont, Texas.

Hertog, M.G. 1996. Epidemiological evi- dence on potential health properties of flavonoids. Proc Nutr Soc. 55 : 385-397.

Hodgson, J.M. 2008. Tea flavonoids and cardiovascular disease. Asia Pac. J. Clin. Nutr. 17(S1) : 288-290.

Hubacek, J.A and D. Bobkova. 2006. Role of cholesterol 7a-hydroxylase (CYP7- A1) in nutrigenetics and pharmaco- genetics of cholesterol lowering. Mol. Diag. Ther. 10 : 93-100.

Hyun-Jung, L., L. Hak-Ju, J. Kang-Hoon, S. Jeing-Kon, L. Seung-Jin and B. Sun-Li. 2006. Compound isolated from Ginkgo biloba bark, isolation method there of and antiplatelet composition containing the same. United States Patent 7341750.

Iswantini, D., D.Nurenda dan P. Sugita.2005. Fraksinasi dan karakterisasi senyawa aktif dari bangle (Zingiber cassumunar Roxb) sebagai aktivator enzim kolesterol oksidase. Prosiding Simposium Nasional Kimia Bahan Alam XV. 13-14 September 2005. Himpunan Kimia Bahan Alam. Bogor.

Kikugawa, K., A. Kunugi and T. Kurechi.2001. Chemistry and implications of degradation of phenolic antioxidants. In Hudson, B.J.F. (ed). Food anti- oxidants. Singapore: Elsevier Applied Modern Toxicology. Mc. Graw-Hill.

Kjeldsen, S.E., A.B. Weder, B. Egan, R.Neubig, A.J. Zweifler and S. Julius.

Effect of circulating epinephrine on platelet function and hematocrit. Hypertension. 25 : 1096-1105.

Koo, S.I. and S.K. Noh. 2007. Green tea as inhibitor of the intestinal absorp- tion of lipids: Potential mechanism for its lipid-lowering effect. J Nutr Biochem. 18 : 179-183.

Leenen, R., A.J. Roodenburg, L.B. Tijburg and S.A.Wiseman. 2005. A single dose of tea with or without milk in- creases plasma antioxidant activity inhumans. Eur. J. Clin. Nutr. 54 : 87-92.

Leung, L.K., Y. Su, R. Chen, Z. Zhang, Y. Huang and Z-Y, Chen. 2001. Theaflavins in black tea and cate- chins in green tea are equally effec- tive antioxidants. J. Nutr. 131 : 2248-2251.

Mandel, S., O.Weinreb, T. Amit and M.B.H. Youdim. 2004. Cell signaling pathways in the neuroprotective ac- tions of the green tea polyphenol (-)

-epigallocatechin-3-gallate: implica- tions for neurodegenerative disea- ses. J. Neurochem. 88: 1555-1569.

Mckenzie, SB. 2004. Clinical Laboratory Hematology. New Jersey : Pearson Education, inc.

Middleton, E. 1998. Effect of plant flavo- noids on immune and inflammatory cell function. Adv. Exp. Med. Biol. 439 : 175-82.

Miliauskas, G., P.R., Venskutonis and T.A. van Beek. 2003. Screening of radical scavenging activity of some medicinal and aromatic plant ex- tracts. Food Chemistry.

Mukamal, K.J., M. Maclure, J.E. Muller, J.B. Sherwood and M.A.Mittleman.

Tea consumption and morta- lity after acute myocardial infarction.

Circulation. 105 : 2476-81.

Murphy, K.J., A.K. Chronopoulos, I. Singh, M.A. Francis, H. Moriarty, M.J. Pike, A.H. Turner, N.J. Mann and A.J. Sinclair. 2003. Dietary flavanols

and procyanidin oligomers from co-coa (Theobroma cacao) inhibit pla- telet function. Am. J. Clin. Nutr. 77:1466-1473.

Pignatelli, P., F. Pulcinelli, A. Celestini, L. Lenti, A. Ghiselli, P.P. Gazzaniaga and F. Violi. 2000. The flavonoids

quercetin and catechin synergistically inhibit platelet function by antago- nizing the intracellular production of hydrogen peroxide. Am. J. Clin. Nutr.

: 1150-1155.

Randox Laboratories. 2009. Cholesterol (Chol) enzymatic endpoint method manual. Available online.

Rein, D., Paglieroni, T.G., Pearson, D.A., Wun, T., Schmitz, H.H., Gosselin, R., and Keen, C.L. 2000. Cocoa and wine polyphenols modulate platelet activa- tion and function. J. Nutr. 130 :2120S-2126S.

Risovic, V., D. Man, O. Sivak, S.D. Lee and K.M. Wasan. 2006. Assessing lipid lowering and plasma cholesteryl ester transfer protein activity of sim- vastatin following administration to rabbits fed a high fat/cholesterol diet. Drug Dev. Ind. Pharm. 32 : 609-615.

Sasazuki, S., H. Kodama, K. Yoshimasu, Y. Liu, M. Washio, K. Tanaka, S. Tokunaga, S. Kono, H. Arai, Y. Doi, T. Kawano, O. Nakagaki, K. Takada, S. Koyanagi, K. Hiyamuta, T. Nii, K. Shirai, M. Ideishi, K. Arakawa, M. Mohri and A. Takeshita. 2000. Rela- tion between green tea consumption and the severity of coronary atheros- clerosis among Japanese men and women. Ann. Epidemiol. 10 : 401-408.

Sheu, J.R., Hsiao G, Chou, P.H, Shen, M.Y. 2004. Mechanisms involved in the antiplatelet activity of rutin, a glycoside of the flavonol quercetin, in

human platelets. J. Agric. Food. Chem. 52 : 4414-4418.

Sesso HD, .M. Gaziano, J.E. Buring and C.H. Hennekens. 1999. Coffee and tea intake and the risk of myocardial infarction. Am. J. Epidemiol. 149 : 162-167.

Tzeng, S.H., W.C. Ko, F.N., Ko and C.M. Teng. 1991. Inhibition of platelet aggregation by some flavonoids. Thrombosis Res. 64 : 91-100.

USDA. 2003. USDA Database for the flavonoid contents of selected foods. Beltsville : US Department of Agri- culture.

Wang, H and K. Helliwell. 2001. Deter- mination of flavonols in green and black tea leaves and green tea infu- sions by HPLC. Food. Res. Inter. 34 :

-227.

Willson, K.C. 1999. Coffee, cocoa and tea. New York : CABI Publishing.

Yang, M.H., C.H., Wang, H.L., and Chen.2001. Green, oolong, and black tea extracts modulate lipid metabolism in hyperlipidemic rats fed high-suc-rose diets. J. Nutr. Biochem. 12 : 14-20.

Yang, C.S and J.M. Landau. 2000. Ef- fects of tea consumption on nutrition and health. J. Nutr. 130 : 2409-2412.

Yung, L.M., F.P. Leung, W.T. Wong, X.Y.Tian, L.H. Yung, Z.Y. Chen, X.Q. Yao, and Y. Huang. 2008. Tea poly- phenols beneft vascular function. In- fammopharmacology. 16 : 230-234.




DOI: http://dx.doi.org/10.21082/bullittro.v22n1.2011.%25p

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