Effect of Container Culture Types on The Success of Cacao Somatic Embryos Formation

Nur Ajijah, Cici Tresniawati, Syafaruddin Syafaruddin

Abstract


Container culture have an important role in determining the success of in vitro culture since it will affect the development of culture, such as the formation of embryonic structures. The study aimed to determine the effect of culture container types on cacao somatic embryogenesis. The study was conducted at Tissue Culture Laboratory, Superior Seed Development Unit of IAARD, Bogor, from April to September 2016. The tests were conducted on the effect of container and explant types as well as the effect of container types and genotypes. The effects of container and explant types were tested using callus induced from petal and staminoid explants of Sca 6, whereas the effects of container types and genotypes were tested using callus induced from petal explants of Sca 6 and ICCRI 4. Afterwards, the somatic embryos were induced using petri dishes or culture bottles according to treatment. The results showed no significant interaction between container and explant types on the average percentage of the formation and number of somatic embryos (10.28% embryos/explants in culture bottles and 7.89% embryos/explants in petri dishes). Meanwhile, there was significant interaction between genotypes and container types in the initial period of somatic embryos formation (15 and 18 weeks after culture), but the effect was not significant in the final period of observation (21 weeks after culture). The results indicate that culture bottles, which have lower prices, can be used to replace petri dishes to induce the formation of somatic embryos in cacao.


Keywords


Container cultures; explants; genotypes; somatic embryos; Theobroma cacao L.

Full Text:

PDF (Indonesian)

References


Ajijah, N. (2016). Pengaruh komposisi media dasar dan jenis eksplan terhadap pembentukan embrio somatik kakao. Jurnal Tanaman Industri dan Penyegar, 3(3), 127–134.

Ajijah, N., & Hartati, R. S. (2016). Pengaruh sitokinin, jenis eksplan, dan genotipe terhadap embriogenesis somatik kakao. Jurnal Tanaman Industri dan Penyegar, 3(2), 71–82.

Ajijah, N., Hartati, R. S., Rubiyo, Sukma, D., & Sudarsono. (2016). Effective cacao somatic embryo regeneration on kinetin supplemented DKW medium and somaclonal variation assessment using SSRs markers. Agrivita, 38(1), 80–92. https://doi.org/10.17503/agrivita.v38i1.619

Ajijah, N., Rubiyo, & Sudarson. (2014). Pembentukan kalus dan embrio somatik kakao menggunakan thidiazuron melalui satu tahap induksi kalus. Jurnal Penelitian Tanaman Industri dan Penyegar, 20(4), 179–186.

Badr-Elden, A. M., Nower, A. A., Ibrahim, A. I., Ebrahim, M. K. H., & Elaziem, T. M. A. (2012). Minimizing the hyperhydricity association with in vitro growth and development of watermelon by modifying the culture conditions. African Journal of Biotechnolgy, 11(35), 8705–8717. https://doi.org/10.5897/AJB11.4276

Chen, C. (2015). Application of growth models to evaluate the microenvironmental conditions using tissue culture plantlets of Phalaenopsis Sogo Yukidian “V3.” Scientia Horticulturae, 191(1), 25-30. https://doi.org/10.1016/j.scienta.2015.05.007

Chunchukov, A., & Yancheva, S. (2015). Micropropagation of paulownia species and hybrids. First National Conference of Biotechnology Vol 100, 223–230. Sofia: Universite de Sofia faculte de Biologie.

de Santana, J. R. F., Paiva, R., de Souza, A. V, & de Oliveira, L. M. (2011). Effect of different culture tube caps and concentrations of activated charcoal and sucrose on in vitro growth and budding induction of Annona glabra L. Cienc. Agrotec, 35(5), 916–923. https://doi.org/10.1016/j.ecoenv.2013.12.022

Fal, M. A., Majada, J. P., & Tames, R. . (2002). Physical environment in non-ventilated culture vessels affects in vitro growth and morphogenesis of several cultivars of Dianthus caryophyllus L . In Vitro Cell. Dev. Biol.Plant, 38, 589–594.

Huang, C., & Chen, C. (2005). Physical properties of culture vessels for plant tissue culture. Biosystems Engineering, 91(4), 501–511. https://doi.org/10.1016/j.biosystemseng.2005.05.005

Islam, T., Dembele, D. P., & Keller, E. R. J. (2005). Influence of explant, temperature and different culture vessels on in vitro culture for germplasm maintenance of four mint accessions. Plant Cell, Tissue and Organ Culture, 81, 123–130.

Prakash, S., Hoque, M., & Brinks, T. (2004). Culture media and containers. Low Cost Options for Tissue Culture Technology in Developing Countries, (February), 29–40. Vienna: International Atomic Energy Agency. https://doi.org/10.1016/j.baae.2007.04.003

Al Shamari, M. (2014). Somatic embryogenesis and cryopreservation of cauliflower (Brassica oleracea var. botrytis)(Doctoral disertation, University of Plymouth).

Takazawa, A., & Kozai, T. (1992). Effect of types of culture vessels with supporting materials on the growth of carnation plantlets in vitro. Environment Control in Biology, 30(2), 65–70. https://doi.org/10.2525/ecb1963.30.65

Tisserat, B., & Silman, R. (2000). Interactions of culture vessels, media volume, culture density, and carbon dioxide levels on lettuce and spearmint shoot growth in vitro. Plant Cell Reports, 19(5), 464–471. https://doi.org/10.1007/s002990050757




DOI: http://dx.doi.org/10.21082/jtidp.v6n2.2019.p89-98

Refbacks

  • There are currently no refbacks.




Copyright (c) 2019 Jurnal Tanaman Industri dan Penyegar

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.


 Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.


 

P-ISSN: 2356-1297
E-ISSN: 2528-7222
Accredited No.30/E/KPT/2018 on Oktober 24, 2018 by Ministry of Research, Technology and Higher Education of the Republic of Indonesia

                    


Jurnal Tanaman Industri dan Penyegar (JTIDP) Editorial Office :

Indonesian Industrial and Beverage Crops Research Institute
Jl. Raya Pakuwon Km. 2, Parungkuda, Sukabumi 43357 Jawa Barat Indonesia
Telp : (0266) 6542181
Fax : (0266) 6542087
Email : jtidp@litbang.pertanian.go.iduppublikasi@gmail.com
Website : http://balittri.litbang.pertanian.go.id



View My Stats