Genetic Variability of 21 Tea Genotypes [Camellia sinensis (L.) O. Kuntze] Based on RAPD Markers

Budi Martono, Syafaruddin Syafaruddin

Abstract


Knowing the genetic diversity in the tea germplasms collection is one of important conditions for assembling new superior varieties. Information of genetic diversity can be obtained through analysis using RAPD molecular markers. The study aimed to determine the genetic diversity of 21 tea genotypes based on RAPD markers. The research was conducted in Integrated Laboratory, Seameo Biotrop, Bogor, from July to September 2013. Genomic DNA was isolated from 21 tea genotypes leaf samples, then amplified with primer OPA 03, OPA 05, OPB 04, OPB 06, OPC 06, and OPD 08. Electrophoresis result was converted into binary data. The genetic similarity and cluster analysis calculation was done using NTSYS-pc version 2.10. In this research, 50 polymorphic bands (94,34%) and 3 monomorphic band (5,66%) were obtained. Cluster analysis based on Nei's genetic distance using the unweighted pair-group method with arithmatic (UPGMA) divided 21 tea genotypes into two groups at a genetic similarity value of 0,48. Group 1 consisted of 20 tea genotypes, while the second group comprised only a one genotype (Sin 27). The range of genetic similarity matrix was between 28%–92%, the lowest genetic similarity (28%) was found between GMB 4 and Sin 27 genotypes, while the highest (92%) was found between AS 2 and AS 1 genotypes. The information obtained can be utilized in breeding programs with the support of agronomic characters as well as in the conservation of tea germplasm.

Keywords


Camellia sinensis; genetic variability; RAPD marker

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References


Astika, W., & Muchtar, D. (1978). Anjuran bahan tanam teh tahun 1978. Warta BPTK, 4(3/4), 297–306.

Boonerjee, S., Islam, M. N., Hoque, M. I., & Sarker, R. H. (2014). Genetic diversity analysis of eighteen tea (Camellia sinensis L.) clones of Bangladesh through RAPD. Plant Tissue Culture and Biotechnology, 23(2), 189–199. http://doi.org/10.3329/ptcb.v23i2.17520

Chen, L., Gao, Q., Chen, D., & Xu, C. (2005). The use of RAPD markers for detecting genetic diversity, relationship and molecular identification of Chinese elite tea genetic resources [Camellia sinensis (L.) O. Kuntze] preserved in a tea germplasm repository. Biodiversity and Conservation, 14(6), 1433–1444. http://doi.org/10.1007/s10531-004-9787-y

Cheng-Wen, S., Yi-Huan, H., Jian-An, H., Jun-Wu, L., Chun-Lin, L., & De-Hua, L. (2008). RAPD analysis on genetic diversity of typical tea populations in Hunan Province. Chinese Journal of Agricultural Biotechnology, 5(1), 67–72. http://doi.org/10.1017/S147923620800199X

Departemen Kehutanan dan Perkebunan. (1998). Kepmenhutbun RI No. 684.c/Kpts-IX/98 tentang pelepasan Teh GPPS 1 sebagai varietas unggul dengan nama GMB 9.

Dinesh, K. P., Shivanna, M. B., & Ram, A. S. (2011). Identification of RAPD (random amplified polymorphic DNA) markers for Ethiopian wild Coffea arabica L. genetic resources conserved in India. IIOABJ, 2(2011), 1–7. Retrieved from http://www.iioab.org/Vol2%284%292011/2%284%291-7.pdf

Direktorat Jenderal Perkebunan. (2017a). Deskripsi varietas benih unggul tanaman perkebunan. Direktorat Jenderal Perkebunan, Kementerian Pertanian.

Direktorat Jenderal Perkebunan. (2017b). Statistik perkebunan Indonesia komoditas teh 2015-2017. Direktorat Jenderal Perkebunan, Kementerian Pertanian.

Goonetilleke, W. A. S. N. S. T., Priyantha, P. G. C., Mewan, K. M., & Gunasekare, M. T. K. (2009). Assessment of genetic diversity of Tea (Camellia sinensis L.O. Kuntze) as revealed by RAPD - PCR markers. Journal of the National Science Foundation of Sri Lanka, 37(2), 147. http://doi.org/10.4038/jnsfsr.v37i2.1072

Kathurima, C. W., Kenji, M. G., Muhoho, M. S., Gichimu, B. M., & Gichuru, E. K. (2012). Genetic diversity among commercial coffee varieties, advanced selections and museum collections in Kenya using molecular markers. International Journal of Biodiversity and Conservation, 4(2), 39–46. http://doi.org/10.5897/IJBC11.231

Kementerian Pertanian. (2018a). Kepmentan RI No. 157/Kpts/KB.010/2/2018 tentang pelepasan varietas Tambi 1 sebagai varietas unggul tanaman teh.

Kementerian Pertanian. (2018b). Kepmentan RI No. 158/Kpts/KB.010/2/2018 tentang pelepasan varietas Tambi 2 sebagai varietas unggul tanaman teh.

Mishra, R. K., Chaudhary, S., Ahmad, A., Pradhan, M., & Siddiqi, T. O. (2009). Molecular analysis of tea clones (Camellia sinensis) using AFLP markers. International Journal of Integrative Biology (Vol. 5).

Martono, B., & Udarno, L. (2014). Keragaman genetik beberapa genotipe teh berdasarkan penanda RAPD (random amplified polymorphic DNA). Jurnal Tanaman Industri Dan Penyegar, 1(1), 1-6. http://doi.org/10.21082/jtidp.v1n1.2014.p1-6

Nei, M. (1972). Genetic distance between populations. The American Naturalist. The University of Chicago Press. The American Society of Naturalists. http://doi.org/10.2307/2459777

Oktavia, F., & Lasminingsih, M. (2011). Selection of parent trees for rubber (Hevea brasiliensis) breeding based on RAPD analysis, 3(3), 2087–3948. http://doi.org/10.13057/nusbiosci/n030304

Panggeso, J., Anshary, A., Samudin, S., & Basri, Z. (2015). Genetic diversity of different cocoa clones by RAPD (random amplified polymorphic DNA) markers. International Journal of Current Research and Academic Review, 3(3), 195–201.

Primrose, S. B., & Twyman, R. M. (2006). Principles of gene manipulation and genomics. American Journal of Human Genetics (7th ed.). Carlton North, Vic: Blackwell. http://doi.org/10.1136/jmg.23.3.282

Pusat Penelitian Teh dan Kina. (2006). Petunjuk kultur teknis tanaman teh. Lembaga Riset Perkebunan Indonesia, Pusat Penelitian Teh dan Kina.

Rahadi, V. P., Khomaeni, H. S., Chaidir, L., & Martono, B. (2016). Keragaman dan kekerabatan genetik koleksi plasma nutfah teh berdasarkan karakter morfologi daun dan komponen hasil. Jurnal Tanaman Industri Dan Penyegar, 3(2), 103–108. http://doi.org/10.21082/jtidp.v3n2.2016.p103-108

Rahmansyah, Mutmainah, Muslimin, & Suwastika, I. N. (2014). Variasi genetik klon kakao (Theobroma cacao L.) di Desa Sausu Peore Kab. Parigi Moutong. Online Jurnal of Natural Science, 3(3), 239–246.

Rohlf, F. J. (2000). NTSYSpc numerical taxonomy and multivariate analysis system user guide. Retrieved from http://www.exetersoftware.com/downloads/ntsysguide21.pdf

Roy, S. C., & Chakraborty, B. N. (2009). Genetic diversity and relationships among tea (Camellia sinensis) cultivars as revealed by RAPD and ISSR based fingerprinting. Indian Journal of Biotechnology, 8, 370–376. Retrieved from http://nopr.niscair.res.in/bitstream/123456789/6141/1/IJBT

Russell, J. R., Hosein, F., Johnson, E., Waugh, R., & Powell, W. (1993). Genetic differentiation of cocoa (Theobroma cacao L.) populations revealed by RAPD analysis. Molecular Ecology, 2(2), 89–97. http://doi.org/10.1111/j.1365-294X.1993.tb00003.x

Shah, S., Yadav, R. N. S., & Borua, P. K. (2015). Molecular analysis by RAPD markers of popular tea (Camellia sinensis) varieties of North-East India infested by tea mosquito bug (Helopeltis theivora). International Journal of Biosciences (IJB), 6(1), 318–325. Retrieved from http://www.innspub.net/wp-content/uploads/2015/01/IJB-V6No1-p318-325.pdf

Shalimol, A., Arumugasamy, K., Kumar, N., Kaffoor, A., Johny, M., & Venugopal, M. (2015). Detection of genetic polymorphisms among commercial tea population and endemic wild tea plant (Gordonia obtusa. Exut and arn) as revealed by RAPD markers. International Journal of Recent Advances in Multidisciplinary Research, 2(7), 543–546. Retrieved from http://www.ijramr.com/sites/default/files/issues-pdf/277_0.pdf

Sriyadi, B., Setiamihardja, R., Baihaki, A., & Astika, W. (2002). Hubungan kekerabatan genetik antar tanaman teh F1 dari persilangan TRI 2024 X PS 1 berdasarkan penanda RAPD. Zuriat, 13(1), 11–20. Retrieved from http://jurnal.unpad.ac.id/zuriat/article/view/6715

Syafaruddin, & Nasution, M. (2012). Keragaman 17 aksesi plasma nutfah kakao berdasarkan penanda morfologi dan molekuler. Buletin RISTRI, 3(2), 177–184.

Venkatachalam, P., Priya, P., Saraswathy-Amma, C. K., & Thulaseedharan, A. (2004). Identification, cloning and sequence analysis of a dwarf genome-specific RAPD marker in rubber tree [Hevea brasiliensis (Muell.) Arg.]. Plant Cell Reports, 23(5), 327–332. http://doi.org/10.1007/s00299-004-0833-8

Venkatachalam, P., Thomas, S., Priya, P., Thanseem, I., Gireesh, T., Saraswathy-Amma, C. K., & Thulaseedharan, A. (2002). Identification of DNA polymorphism among clones of Hevea brasiliensis Muell. Arg. Using RAPD analysis. Indian J Nat Rubb Res, 15(2), 172–181.

Wang, X. F., Zheng, H. Y., Zheng, W. H., Ao, C. Q., Jin, H. Y., Zhao, L. H., … Jia, L. R. (2011). RAPD-based genetic diversities and correlation with morphological traits in Camellia (Theaceae) cultivars in China. Genetics and Molecular Research, 10(2), 849–859. http://doi.org/10.4238/vol10-2gmr1207

Yamanishi, T. (1991). Tea flavor: Global Advances in Tea Science. (N. K. Jain, Ed.). New Delhi: Aravali Book International (P) Ltd.

Zewei, A., Han, C., Aihua, S., Jianlin, F., & Huasun, H. (2005). Identification of rubber clones by RAPD markers. In International Natural Rubber Conference.




DOI: http://dx.doi.org/10.21082/jtidp.v5n2.2018.p77-86

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