Effect of Plant Oils and Extracts on Uredospores of Hemileia vastatrix Germination and Attack Intensity

Rita Harni, Efi Taufik, Samsudin Samsudin

Abstract


Rust disease caused by fungus Hemileia vastatrix is a major disease of Arabica coffee, which reduces yield by 20%–70%. Botanical fungicide is a potential alternative because environmentally friendly and safe to humans health. The research aimed to analyze the effect of oils and extracts of fungicidal plants on uredospore germination and attack intensity of H. vastatrix. The research was conducted in laboratory and greenhouse of Indonesian Industrial and Beverages Crops Research Institute (IIBCRI), Sukabumi, from January to December 2016. The plant oils were of cloves, citronella, Reutealis trisperma, and neem, while the plant extracts used were mahogany, Ageratum conyzoides, and wood vinegar. Those oils and extracts were assessed on uredospores germination of H. vastatrix, both in vitro and on coffee seedlings in the greenhouse. A complete randomized block design was used with 9 treatments and 5 replications. The oils and extracts at 5% concentration were applied on coffee leaves of 6 months old plants then inoculated with H. vastratrix uredospora simultaneously. Attack symptoms, incubation period, attack percentage and intensity were observed. The results showed that plant oils and extracts used in present study effectively reduced the uredospora germination of H. vastatrix. However, oils of neem and R. trisperma as well as A. conizoides extract are more potential to suppress H. vastatrix infection in coffee leaves in greenhouse and reduced attack intensity from 22.2% to 3.6%; 5.2%; and 7.6% with inhibitory level at 83.8%; 76.6%; and 65.8%, respectively. Therefore, they are considered as potential biocontrols for rust disease.

Keywords


H. vastatrix; plant extract; plant oil; rust leaf disease

Full Text:

PDF (Indonesian)

References


Agrios, G. N. (2005). Plant pathology. Fifth Edition. New York: Elsevier Academic Press. http://doi.org/ 10.1017/CBO9781107415324.004

Al-Hazmi, R. H. M. (2013). Effect of Neem (Azadirachta indica) leaves and seeds extract on the growth of six of the plant disease causing fungi. Global Advanced Research Journal of Microbiology, 2(5), 2315–5116.

Brown, J. S., Whan, J. H., Kenny, M. K., & Merriman, P. R. (1995). The effect of coffee leaf rust on foliation and yield of coffee in Papua New Guinea. Crop Protection, 14(7), 589–592. http://doi.org/ 10.1016/0261-2194(95)00040-2

Budiani, A., Susanti, I., Mawardi, S., Santoso, D.A., & Siswanto. (2004). Ekspresi β-1,3 glukanase dan kitinase pada tanaman kopi arabika (Coffea arabica L.) tahan dan rentan karat daun. Menara Perkebunan, 72(2), 57–70.

Castillo-Z, J. (1989). Breeding for rust resistance in Colombia. In Coffee Rust: Epidemiology, resistance and management (pp. 307–316). Florida: CRC Press, Inc.

de Billerbeck, V. G., Roques, C. G., Bessière, J. M., Fonvieille, J. L., & Dargent, R. (2001). Effects of Cymbopogon nardus (L.) W. Watson essential oil on the growth and morphogenesis of Aspergillus niger. Canadian Journal of Microbiology, 47(1), 9–17. http://doi.org/10.1139/cjm-47-1-9

Deng, J., Li, W., Peng, X., & Hao, X. H. (2013). Research Article Study on the potential of antifungal activity of essential oils against fungal pathogens of fruits and vegetables. J. Chem. Pharm. Res., 5(12), 443–446.

Ekowati, N., Sucianto, E. T., Muljowati, J. S., & Dewi, R. . (2009). Uji aktivitas antibiosis beberapa isolat Gliocladium dan Trichoderma terhadap mikroba patogen dengan pH awal fermentasi yang berbeda. Jurnal Inovasi, 3(2), 69–77.

El-Zemiti, S. R., & Ahmed, S. M. (2005). Antifungal activity of some essential oils and their major chemical constituents against some phytopathogenic fungi. J. Pest. Cont. & Environ. Sci., 13(1), 61–72.

Eskes, A. B., & Toma-Braghini, M. (1981). Assesment methods for resistance to coffee rust (Hemileia vastatrix B et Br.). Pl. Prot. Bull. FAO, 29, 56–66. Retrieved from agris.fao.org/agris-search/ search.do?recordID=US201301988652

Ginting, C. (2006). Perkecambahan uredospora Hemileia Vastatrix. J. HPT Tropika, 6(1), 52–58.

Harni, R., Amaria, W., & Supriadi. (2013). keefektifan beberapa formula fungisida nabati eugenol dan sitronella terhadap Phytopthora palmivora Bult. Asal Kakao. Jurnal Tanaman Industri Dan Penyegar, 4(1), 11–18. http://doi.org/10.21082/ jtidp.v4n1.2013. p11-18

Harni, R., & Baharuddin, B. (2014). Keefektifan Minyak Cengkeh, Serai Wangi, dan Ekstrak Bawang Putih terhadap Penyakit Vascular Streak Dieback (Ceratobasidium theobromae) Pada Kakao. Jurnal Tanaman Industri Dan Penyegar, 1(3), 167–174. http://doi.org/10.21082/jtidp.v1n3.2014.p167-174

Harni, R., & Randriani, E. (2016). Penyakit karat daun pada beberapa varietas kopi Arabika di kabupaten Garut. Medkom Perkebunan Tanaman Industri Dan Penyegar, 4(7), 66.

Harni, R., Taufiq, E., & Martono, B. (2015). Ketahanan pohon induk kopi Liberika terhadap penyakit karat daun (Hemileia vastatrix B. Et Br.) di Kepulauan Meranti. J. Tidp, 2(1), 35–42. http://doi.org/ 10.21082/jtidp.v2n1.2015.p35-42

Helal, G. A., Sarhan, M. M., Abu Shahla, A. N. K., & Abou El-Khair, E. K. (2007). Effects of Cymbopogon citratus L. essential oil on the growth, morphogenesis and aflatoxin production of Aspergillus flavus ML2-strain. Journal of Basic Microbiology, 47(1), 5–15. http://doi.org/10.1002/jobm.200610137

Javed, S., & Bashir, U. (2012). Antifungal activity of different extracts of Ageratum conyzoides for the management of Fusarium solani. African Journal of Biotechnology, 11(49), 11022–11029. http://doi.org /10.5897/AJB12.366

Knobloch, K., Pauli, A., Iberl, B., Weigand, H., & Weis, N. (1989). Antibacterial and antifungal properties of essential oil components. Journal of Essential Oil Research, 1(3), 119–128. http://doi.org/10.1080 /10412905.1989.9697767

Liu, X., Wang, L. P., Li, Y. C., Li, H. Y., Yu, T., & Zheng, X. D. (2009). Antifungal activity of thyme oil against Geotrichum citri‐aurantii in vitro and in vivo. Journal of Applied Microbiology, 107(5), 1450–1456. http://doi.org/10.1111/j.1365-2672.2009.04328.x

Medice, R., Alves, E., de Assis, R. T., Magno Júnior, R. G., & Lopes, E. A. das G. L. (2007). Óleos essenciais no controle da ferrugem asiática da soja Phakopsora pachyrhizi Syd. & P. Syd. Ciência E Agrotecnologia, 31(1), 83–90. http://doi.org/10.1590/S1413-70542007000100013

Moslem, M., & El-Kholie, E. (2009). Effect of Neem (Azardirachta indica A. Juss) seeds and leaves extract on some plant pathogenic fungi. Pakistan Journal of Biological Science, 12(14), 1045–1048. Retrieved from http://docsdrive.com/pdfs/ansinet/pjbs/ 2009/ 1045-1048.pdf

Nakahara, K., Alzoreky, N. S., Yoshihashi, T., Nguyen, H. T. T., & Trakoontivakorn, G. (2003). Chemical composition and antifungal activity of essential oil from Cymbopogon nardus (Citronella Grass). Japan Agricultural Research Quarterly, 37(4), 249–252. http://doi.org/10.6090/jarq.37.249

Pereira, R. B., Lucas, G. C., Perina, F. J., & Alves, E. (2012a). Essential oils for rust control on coffee plants. Ciência E Agrotecnologia, 36(1), 16–24. http://doi.org/10.1590/S1413-70542012000100002

Perello, A., Noll, U., & Slusarenko, A. J. (2013). In vitro efficacy of garlic extract to control fungal pathogens of wheat. Journal of Medicinal Plants Research, 7(24), 1809–1817. http://doi.org/10.5897/jmpr12.511

Piper, P., Calderon, C. O., Hatzixanthis, K., & Mollapour, M. (2001). Weak acid adaptation: The stress response that confers yeasts with resistance to organic acid food preservatives. Microbiology, 147(10), 2635–2642. http://doi.org/10.1099/00221287-147-10-2635

Pusat Penelitian Kopi dan Kakao. (2011). Seratus Tahun Pusat Penelitian Kopi dan Kakao Indonesia 1911-2011. Pusat Penelitian Kopi dan Kakao.

Rasooli, I., Rezaei, M. B., & Allameh, A. (2006). Growth inhibition and morphological alterations of Aspergillus niger by essential oils from Thymus eriocalyx and Thymus x-porlock. Food Control, 17(5), 359–364. http://doi.org/10.1016/J.FOODCONT. 2004.12.002

Semangun, H. (2008). Penyakit-penyakit Tanaman Perkebunan Di Indonesia. Gadjah Mada University Press. Retrieved from http://ugmpress.ugm.ac.id /id/product/teknologi-pertanian/penyakit-penyakit-tanaman-perkebunan-di-indonesia

Soesanthy, F., & Samsudin. (2014). Pengaruh Beberapa Jenis Formula Insektisida Nabati untuk Melindungi Buah Kakao dari Serangan Penggerek‏. Jurnal Tanaman Industri Dan Penyegar‏, 1(2), 69–78.

Vogt, T. (2010). Phenylpropanoid biosynthesis. Molecular Plant, 3(1), 2–20. http://doi.org/10.1093/mp /ssp106




DOI: http://dx.doi.org/10.21082/jtidp.v5n2.2018.p67-76

Refbacks

  • There are currently no refbacks.




Copyright (c) 2018 Jurnal Tanaman Industri dan Penyegar

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.


 Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.


 

P-ISSN: 2356-1297
E-ISSN: 2528-7222
Accredited No.699/AU2/P2MI-LIPI/10/2015 on Oktober 30, 2015 by Indonesian Institute of Science

                    


Jurnal Tanaman Industri dan Penyegar (JTIDP) Editorial Office :

Indonesian Industrial and Beverage Crops Research Institute
Jl. Raya Pakuwon Km. 2, Parungkuda, Sukabumi 43357 Jawa Barat Indonesia
Telp : (0266) 6542181
Fax : (0266) 6542087
Email : jtidp@litbang.pertanian.go.iduppublikasi@gmail.com
Website : http://balittri.litbang.pertanian.go.id



View My Stats