IMPROVEMENT OF CALLUS FORMATION OF CASHEW IN VITRO CULTURE FROM LEAF AND FLOWER PETAL EXPLANTS

Nur Ajijah, Rossa Yunita

Abstract


The increasing of the callus formation of cashew (Anacardium occidentale L.) from leaf explants and the petals have successfully done through the use of a combination of plant growth regulators 2,4-D, pocloram and BA. On leaf explants the highest percentage of callus formation was obtained in combination treatment of plant growth regulators 2,4-D 5 mg/l + BA 1 mg/l and 2,4-D 5 mg/l + picloram 0.5 mg/l with callus formation percentage of 33.3%. On the use of petal explants the highest percentage of callus formation was obtained on 2,4-D treatment of 10 mg/l with callus formation percentage of 40%. Formed callus were blackish brown demonstrate the use of PVP 200 mg/l still can not overcome the problem of phenol oxidation.

Keywords


Anacardium occidentale L.; callus; leaves, petals

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 Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.


 

P-ISSN: 2356-1297
E-ISSN: 2528-7222
Accredited No.30/E/KPT/2018 on Oktober 24, 2018 by Ministry of Research, Technology and Higher Education of the Republic of Indonesia

                    


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