The Effectiveness of Biofungicide Trichoderma sp. with Three Kinds of Carrier on White Root Disease Rigidoporus microporus

Widi Amaria, Funny Soesanthy, Yulius Ferry

Abstract


The effectiveness of Trichoderma sp as biocontrol for white root disease is determined by the environment, thus it is best applied as biofungicide. The application of Trichoderma sp. with suitable carrier is expected to suppress pathogen (Rigidoporus microporus) in rubber seedlings. The objective of the research was to study the effectiveness of Trichoderma sp. biofungicide with three types of carriers in controlling white root disease in rubber seedlings. The research was conducted in Plant Protection Laboratory and screen house of Indonesian Industrial and Beverage Crops Research Institute (IIBCRI), Sukabumi, from July to December 2013. The experiment used a factorial design with 2 factors and 3 replications. The first factor is 4 types of Trichoderma, namely Trichoderma virens, T. hamatum, T. amazonicum, dan T. atroviride whereas the second factor is the three types of carrier, namely molasses, compost, and talc. The biofungicide were made of four species of Trichoderma and the three carriers thus resulting in 12 biofungicides. The spora population of Trichoderma sp. was 108 spores/ml from which then 100 ml or gram applied on each plant.  Rubber seedlings used were of AVROS 2037 clones, 3 months old clone-grafted seedlings grown in polybag. Observations were on the incubation stage, the intensity of the white root disease attack, the disease suppression, and the population of Trichoderma sp. in soil. The results showed no interactions between types of Trichoderma with types of carrier. The four Trichoderma species studied had similar suppressing effectivity on white root disease in rubber seedlings.  Talc, compost, and molasses increased the Trichoderma sp.  growth but talc was shown had the highest effectivity in suppressing the disease. 

Keywords


Rubber; white root disease; biofungicide; Trichoderma sp.

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References


Amaria, W., Taufiq, E., & Harni, R. (2013). Seleksi dan identifikasi jamur antagonis sebagai agens hayati jamur akar putih Rigidoporus microporus pada tanaman karet. Buletin Riset Tanaman Rempah dan Aneka Tanaman Industri, 4(1), 55–64. doi: http://dx.doi.org/ 10.21082/jtidp.v4n1.2013.p55-64.

Amaria, W., & Wardiana. (2014). Pengaruh waktu aplikasi dan jenis Trichoderma terhadap penyakit jamur akar putih pada bibit tanaman karet. Jurnal Tanaman Industri dan Penyegar, 1(1), 45–54. doi: http:// dx.doi.org/10.21082/jtidp.v1n2. 2014.p79-86.

Amaria, W., Harni, R., & Samsudin. (2015). Evaluasi jamur antagonis dalam menghambat pertumbuhan Rigidoporus microporus penyebab penyakit jamur akar putih pada tanaman karet. Jurnal Tanaman Industri dan Penyegar, 2(1), 235–244. doi: http://dx.doi.org/ 10.21082/jtidp.v2n1.2015. p51-60

Benítez, T., Rincón, A.M., Limón, M.C., Codón, A.C. (2004). Biocontrol mechanisms of Trichoderma strains. Int Microbiol., 7(4), 249–60.

Berlian, I., Setyawan, B., & Hadi, H. (2013). Mekanisme antagonisme Trichoderma spp. terhadap beberapa patogen tular tanah. Warta Perkaretan, 32(2), 74–82.

Boggie, L. M., & Person, H. (1988). Plant roots and their environment. Development in agricultural and manage forest. Uppsala Sweden.

Damiri, N., Mulawarman, & Mutiara, M. (2014). Effect of temperature and storage on effectiveness of Trichoderma viride as biocontrol agents of Rigidoporus microporus, Pathogen of white root on rubber. AGRIVITA, 36(2), 169–173. Retrieved from http://dx.doi.org/10.17503/Agrivita-2014-36-2-p169-173.

Druzhinina, I. S., Seidl-Seiboth, V., Herrera-Estrella, A., Horwitz, B.A., Kenerley, C.M., Monte, E., … Kubicek, CP. (2011). Trichoderma: the genomics of opportunistic success. Nature Reviews Microbiology, 9, 749–759. doi: 10.1038/nrmicro2637.

Fairuzah, Z., Dalimunthe, C.I., Karyudi, Suryaman, S., & Widhayati, W. (2012). Efektivitas endohevea dalam mengendalikan penyakit jamur akar putih pada tanaman karet. Prosiding Konferensi Nasional Karet (pp. 259–268). Yogyakarta, 19–20 September 2012.

Gupta, V.K., SchMoll, M., Herrera-EStrella, A., Upadhyay, R.S., Druzhinina, I., & Tuohy, M.G. (2014). Biotechnology and biology of Trichoderma (p. 527). Amsterdam, Netherlands: Elsevier B.V.

Hidayat, R. W. (2013). Produksi biofungisida Trichoderma harzianum pada berbagai media cair untuk mengendalikan penyakit lanas tembakau (Phytophthora nicotianae) (Skripsi, Program Studi Agroteknologi Fakultas Pertanian Universitas Jember, Jember).

Jayasinghe, C. K. (2010). White root disease of rubber tree: An overview. Proceedings of International Workshop on White root rot disease of Hevea Rubber (pp. 1–8). 14th–16th Dec. 2010, Hotel Janaki, Colombo, Sri-Lanka.

Jeyarajan, R., & Nakkeeran, S. (2000). Exploitation of microorganisms and viruses as biocontrol agents for crop disease mangement. In Biocontrol Potential and their Exploitation in Sustainable agriculture (pp. 95–116). Upadhyay et al. (Ed.), Kluwer Academic/ Plenum Publishers, USA. Retrieved from http://dx.doi. org/10.1007/978-1-4615-4209-4_8.

Mukherjee, M., Mukherjee, P.K., Horwitz, B.A., Zachow, C., Berg, G. Zeilinger, S. (2012). Trichoderma–plant–pathogen interactions: Advances in genetics of biological control. Indian J Microbiol, 52(4), 522–529. doi: 10.1007/s12088-012-0308-5.

Mukherjee, P. K., Horwitz, B. A., Herrera-Estrella, A., Schmoll, M., & Kenerley, C. M. (2013). Trichoderma research in the genome era. Annu. Rev. Phytopathol., 51, 105–29.

Nurhayatiningsih. (2013). Prospek jamur Trichoderma koningii untuk pengendalian penyakit Phytophthora palmivora pada tanaman kakao. Retrieved from http://ditjenbun.pertanian.go.id/bbpptpsurabaya/tinymcpuk/gambar/file/prospek%20trico%20tuk%20Kakao.pdf

Omorusi, V. I. (2012). Effects of white root rot disease on Hevea brasiliensis (Muell. Arg.)-Challenges and Control Approach. (pp. 139-152). Retrieved from http://dx.doi.org/ 10.5772/ 54024.

Panahian, Gh., Rahnama, K., & Jafari, M. (2012). Mass production of Trichoderma spp. and application. International Research Journal of Applied and Basic Sciences, 3(2), 292–298.

Patel, R., & Patel, D. (2014). Screening of Trichoderma and antagonistic analysis of a potential strain of Trichoderma for production of a bioformulation. International Journal of Scientific and Research Publications, 4(10), 1–6.

Semangun, H. (2000). Penyakit-penyakit tanaman perkebunan Indonesia (p. 835). Yogyakarta: Gadjah Mada University Press.

Setyorini, D., Saraswati, R., & Anwar, E.K. (2006). Kompos. In Simanungkalit, R.D.M., Suriadikarta, D.A., Saraswati, R., Setyorini, D., & Hartatik, W. (Eds), Pupuk organik dan pupuk hayati. Bogor: Balai Besar Litbang Sumberdaya Lahan Pertanian Badan Penelitian dan Pengembangan Pertanian.

Sharma, Radheshyam, Joshi, A., & Dhaker, R. C. (2012). A brief review on mechanism of Trichoderma fungus use as biological control agents. International Journal of Innovations in Bio-Sciences, 2(4), 200–210.

Sinulingga, W., & Eddy. (1989). Pengendalian penyakit jamur akar putih pada tanaman karet (pp. 8–15). Medan: Pusat Penelitian Sungei Putih.

Situmorang, A. (2004). Status dan manajemen pengendalian penyakit akar putih di perkebunan karet. In Sitomurang, A., Budiman, A., Suryaningtyas, H., Thomas, Lasminingsih, M., & Gunawan, A. (Eds). Pertemuan Teknis Strategi Pengelolaan Penyakit Tanaman Karet untuk Mempertahankan Potensi Produksi Mendukung Industri Perkaretan Indonesia Tahun 2020 (pp. 66–86). Palembang, 6–7 Oktober 2004.

Sriram, S., Roopa, K. P., & Savitha, M. J. (2011). Extended shelf-life of liquid fermentation derived talc formulations of Trichoderma harzianum with the addition of glycerol in the production medium. Crop Protection, 30, 1334-1339.

Subash, N., Meenakshisundaram, M., Sasikumar, C., & Unnamalai, N. (2014). Mass cultivation of Trichoderma harzianum using agricultural waste as a substrate for the management of damping off disease and growth promotion in chilli plants (Capsicum annuum L.). International Journal of Pharmacy and Pharmaceutical Sciences, 6(5), 188–192.

Suwandi. (2008). Evaluasi kombinasi isolat Trichoderma mikoparasit dalam mengendalikan penyakit akar putih pada bibit karet. J. HPT Tropika, 8(1), 55–62.

Wijesinghe, C.J., Wilson Wijeratnam, R.S., Samarasekara, J.K.R.R., Wijesundera, R.L.C. (2011). Development of a formulation of Trichoderma asperellum to control black rot disease on pineapple caused by (Thielaviopsis paradoxa). Crop Protection, 30, 300–306.




DOI: http://dx.doi.org/10.21082/jtidp.v3n1.2016.p37-44

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