Effect of Endopytic Bionematicide Bacillus sp. on The Infection of Meloidogyne sp. of Coffee Plant

Rita Harni, Samsudin Samsudin

Abstract


Meloidogyne sp. is an important pathogen of coffee plant.  Bacillus sp. is commonly used to control the nematode as it is environmentally friendly. The study aimed to determine the effect of endophytic bacteria Bacillus sp. PG76 formulated in the molasses, talc or compost on the infection of Meloidogyne sp. The experiments were conducted in the laboratory and greenhouse of the Indonesian Industrial and Beverage Crops Research Institute, Sukabumi from December 2013 to May 2014. The experimental design was a completely randomized, 6 treatments and 10 replications of six months-old coffee plants. Six treatments were tested (Bacillus sp. PG76 formulated in molasses, compost, or talc; carbofuran; plant inoculated with the nematode only, and plant neither treated with the formula nor the nematode).  Population of Bacillus sp. PG76 in each formula was 109 cfu/ml. Number of formulas applied per plant was 100 ml of molasses, 100 g of talc, or 100 g of compost.  One week after the treatments, the plants were inoculated with 500 larvae-2 Meloidogyne sp. Parameters observed were the number of gall, nematode population in the roots and soil, and the coffee plant growth.  The results showed that all the treatments (molasses, compost, and talc) suppressed the population of Meloidogyne sp.  The best formulas were molasses and compost that reduced the nematode infection up to 74.0% and 73.2%, respectively, similar to that of carbofuran (i.e. 73.3%). Furthermore, application of the formulas increased coffee plant growth. The study suggests that Bacillus sp. PG76 formulation is prospective to control the nematode. 

Keywords


Bacillus sp.; Meloidogyne sp.; molasses; compost; talc

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References


Aira, M., Monroy, F., & Dominguez, J. (2006). Changes in microbial biomass and microbial activity of pig slurry after the transit through the gut of the earthworm Eudrilus eugeniae. Biol. Fertil Soil, 42, 371–376.

Aisyah, I.N., Wiryadiputra, S., Fauzi, I., & Harni, R. (2015). Populasi Pratylenchus coffeae (Z) dan pertumbuhan bibit kopi Arabika akibat inokulasi Pseudomonas diminuta L. dan Bacillus subtilis (C.). Pelita Perkebunan, 31(1), 30–40.

Bacon, C.W., & Hinton, S. S. (2007). Bacterial endophytes: The endophytic nische, its occupants, and its utility. In Gnanamanickam, S.S. (Ed.), Plant-associated bacteria (pp. 155–194). Berlin: Springer.

Cook, R.J., & Baker, K.F. (1983). The nature and practice of biological control of plant pathogens. USA: The American Phytopathol. Soc.

Hallmann, J., & Berg, G. (2006). Spectrum and population dynamics of bacterial root endophytes. In Schulz, B., Boyle, C., & Sieber, T. (Eds), Soil biology microbial root endophytes Vol. 9 (pp. 15–31). Berlin, Heidelberg: Springer.

Hanudin, W., Nuryani, E., Silvia Yusuf, & Marwoto, B. (2011). Biopestisida organik berbahan aktif Bacillus substilis dan Pseudomonas fluorescens untuk mengendalikan penyakit layu fusarium pada anyelir. J. Hort., 21(2), 152–163.

Harni R. (2010). Pengaruh bakteri endofit untuk menginduksi ketahanan tanaman nilam terhadap infeksi nematoda peluka akar (Pratylenchus brachyurus). Laporan Hasil Penelitian Riset Unggulan Terpadu. Jakarta: Kementerian Riset dan Teknologi.

Harni, R. (2012). Pemanfaatan bakteri endofit untuk mengendalikan nematoda Pratylenchus coffeae dan Radhopolus similis pada tanaman kopi. Laporan Hasil Penelitian. Sukabumi: Balai Penelitian Tanaman Industri dan Penyegar.

Harni R., & Munif, A. (2012). Pemanfaatan agens hayati endofit untuk mengendalikan penyakit kuning pada tanaman lada. Buletin Riset Tanaman Rempah dan Aneka Tanaman Industri, 3(3), 201–206.

Harni R, Supramana, M.S., Sinaga, Giyanto, & Supriadi. (2012). Mekanisme bakteri endofit mengendalikan nematoda Pratylenchus brachyurus pada tanaman nilam. Bulletin Spices and Medicinal Crops, 23(1), 102–114.

Harni, R., & Khaerati. (2013). Evaluasi bakteri endofit untuk mengendalikan nematoda Pratylenchus coffeae pada tanaman kopi. Buletin Riset Tanaman Rempah dan Aneka Tanaman Industri, 4(2), 109–116.

Harni, R. (2014). Pengaruh beberapa isolat bakteri endofit terhadap nematoda puru akar (Meloydogyne spp.) pada tanaman kopi. In Prosiding Perlindungan Tanaman II, Bogor: Institut Pertanian Bogor.

Harni, R., Supraman, & Supriadi. (2014). Eficacy of endophytic bacteria in reducing plant parasitic nematode Pratylenchus brachyurus. Indonesian Journal of Agricultural Science, 15(1), 29–34.

Hasky-Gunther, K., Hoffmann-Hergarten, S., & Sikora, R.A. (1998). Resistance against the potato cyst nematode Globodera pallida systemically induced by the rhizobacteria Agrobacterium radiobacter (G12) and Bacillus sphaericus (B43). Fundam. Appl. Nematol., 21(5), 511–517.

Hrynkiewiezc, K., & Baum, C. (2012). The potential of rhizosphere microorgamisms to promote the plant growth in disturbed soils. In Malik A., & Grohmann (Eds). Environmental protection strategies for sustainable development. USA.

Jayarajan, R., & Nakkeeran, S. (2000). Exploitation of microorganisms and viruses as biocontrol agents for crop disease management. In Biocontrol Potential and Their Exploitation in Sustainable Agriculture (pp. 95-116). USA: Kluwer Academic Publishers.

Kloepper, J.W., Ryu, C.M., & Zhang, S. (2004). Induced systemic resistance and promotion of plant growth by Bacillus spp. Phytopathological, 94(11), 1259–1266.

Lamovsek, J., Urek, G., & Trdan, S., (2013). Biological control of root-knot nematodes (Meloidogyne spp.): Microbes against the pests. Acta agriculturae Slovenica, 101, 263–275.

Mankau, R. (1981). Microbial control nematodes. In Plant parasitic nematodes. Vol. 3 (pp. 475–494).

Mekete, T., Hallmann, J., Sebastian, K., & Sikora, R. (2009). Endophytic bacteria from Ethiopian coffee plants and their potential to antagonisme Meloidogyne incognita. Nematology, 11(1), 117–127.

Munif, A., Hallmann, J., & Sikora, R.A. (2013). The influence of endophytic bacterie on Meloidogyne incognita infection and tomato plant growth. J. ISSAAS, 19(2), 68–74.

Nakkeeran, S., Fernando, W.G.D., & Siddiqui, Z.A. (2005). Plant growth promoting rhizobacteria formulations and its scope in commercialization for the management of pests and diseases. In Siddiqui, Z.A. (Ed.), PGPR: Biocontrol and biofertilization (pp. 257–296). Dordrect, The Netherland: Springer.

Nawangsih, A.A., Wijayanti, E., & Kartika, J.G. (2014). Pengembangan formulasi biopestisida berbahan aktif bakteri endofit dan PGPR untuk mengendalikan penyakit layu bakteri. In Strategi Perlindungan Tanaman dalam Memperkuat Sistem Pertanian Menghadapi ASEAN Free Trade Area (AFTA) dan ASEAN Economic Community (AEC) 2015. Prosiding Seminar Nasional Perlindungan Tanaman II (pp. 97-103). Bogor, 13 November 2014.

Nusantara, A.D., Mansyur, I., Kuswana, C., Darusman, L.K., & Soedarmadi. (2007). Peran subtrat alami, kadar air, dan sterilisasi dalam produksi spora melalui simbiosis Pueraria javanica dan Glomus etunicatum. J. Akta Agrosia., 2, 204–212.

Rahanandeh, H., Khodakaramian, G., Seraji, A., Asghari, S.M., & Tarang, A.R. (2012). Inhibition of tea root lesion nematode Pratylenchus loosi.

Siddiqui, I.A., & Shaukat, S. S. (2004). Systemic resistance in tomato induced by biocontrol bacteria against the root-knot nematode, Meloidogyne javanica is independent of salicylic acid production. J. Phytopathology, 152, 48–54.

Souza, R.M. (2008). Plant-parasitic nematodes of coffee (pp. 225–248). Springer.

Vetrivelkalai, P., Sivakumar, M., & Jonathan, E. I. (2010). Biocontrol potential of endophytic bacteria on Meloidogyne incognita and its effect on plant growth in bhendi. Journal of Biopesticides, 3(2), 452–457.




DOI: http://dx.doi.org/10.21082/jtidp.v2n3.2015.p143-150

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P-ISSN: 2356-1297
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