Potency of Rhizobacteria and Botanical Fungicides to Control White Root Fungus Disease in Rubber Plant

Nasrun Nasrun, Nurmansyah Nurmansyah

Abstract


The white root fungus (WRF) disease caused by Rigidoporus microporus is the main disease that reduce rubber production. The use of biological agents, such as rhizobacteria and botanical fungicide is expected to control WRF disease. The research aimed to determine the potential of rhizobacteria and botanical fungicide with the active compound of citronellal, geraniol, eugenol, and catechin to suppress the growth of R. microporus and control WRF disease. The in vitro studies were conducted at the laboratory of KP. Laing, Solok, whereas in planta tests at rubber plantations in Sijunjung, West Sumatra from April to November 2012. The in planta tests used the factorial designs, which were arranged in a randomized complete block design with 2 factorials and 3 replications. The first factor was 4 treatments, using Bacillus sp. Bc94, Pseudomonas fluorescens Pf55, a combination of Bacillus sp. Bc94 and P. fluorescens Pf55, and without rhizobacteria. The second factor was 3 treatments, using biofungicide formula: formula F1, formula F2, and without botanical fungicide. The parameters assessed were inhibition of R. microporus growth and  development of WRF disease. The results showed that Bacillus sp. Bc94, P. fluorescence Pf55 and the combination of citronellal, geraniol, eugenol, and catechin had the highest inhibitions on R. microporus growth: 89.54%, 90.49%, and 81.39%, respectively. The combinations of Bacillus sp. Bc94 and P. fluorescens PF55 with botanical fungicide formulas (F1 and F2) significantly inhibited the WRF disease intensity up to 80.95%–82.91%.


Keywords


Rubber; Rigidoporus microporus; white root fungus; Rhizobacteria; botanical fungicide

Full Text:

PDF (Indonesian)

References


Akila, R., Rajendran, L., Harish, S., Saveetha, K., Raguchanderand, T., & Samiyappan, R. (2011). Combined application of botanical formulations and biocontrol agents for the management of Fusarium oxysporum f. sp. cubense (Foc) causing Fusarium wilt in banana. Tamil Nadu, India: Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Lawley Road, Coimbatore 641 003.

Arwiyanto, T. (1998). Pengendalian secara hayati penyakit layu bakteri pada tembakau. Laporan Riset Unggulan Terpadu IV (1996-1998) (p. 58). Jakarta: Kantor Menteri Negara Riset dan Teknologi Dewan Riset Nasional.

Beneduzi, A, Ambrosini, A., & Passaglia, L.M.P. (2012). Plant growth-promoting rhizobacteria (PGPR): Their potential as antagonists and biocontrol agents. Genetics and Molecular Biology, 35(4), 1044–1051.

Butelho, G.R., & Mendonca-Hagler, L.C. (2006). Fluorescent Pseudomonads associated with the rhizosphere of crops-An overview. Brazilian Journal of Microbiology, 37, 401–416.

Bull, C.T., Weller, D.M., & Thomashow, L.S. (1991). Relation between root colonization and suppression of Gaeumannomyces graminis var. tritici by Pseudomonas fluorescens strain 2-79. Phytophatology, 81, 954–959.

Campbell, R. (1989). Biological control of microbial plant pathogens (p. 218). Cambridge: Cambridge University Press.

Chrisnawati, Nasrun, & Triwidodo, A. (2009). Pengendalian penyakit layu bakteri nilam menggunakan Bacillus spp dan Pseudomonas fluorescens. Jurnal Penelitian Tanaman Industri, 15(3), 116-123.

Devi, S.I., Talukdar, N.C., Sharma, K.C., Jeyaram, K., & Rohinikumar, M. (2011). Screening of rhizobacteria for their plant growth promotion ability and antagonism against damping-off and root rot disease of broad bean (Vicia faba L). Indian J. Microbiol, 51(1), 14–21.

Djiwanti, S.R., & Wiratno. (2011). Evaluasi pemanfaatan formula pestisida nabati cengkeh dan seraiwangi untuk pengendalian busuk rimpang jahe (pp. 213-222). Semnas Pesnab IV. Jakarta, 15 Oktober 2011.

Emilda, D., & Istianto, M. (2011). Pengaruh minyak cengkeh terhadap pertumbuhan koloni dan sifat antagonis cendawan Gliocladium sp. terhadap Fusarium oxysporum f.sp. cubense. J. Hort., 21(1), 33–39.

Ezzat, S.M. (2014). Biocontrol of phytopathogenic bacteria isolated from drainage water and causing bacterial blight disease. World Applied Sciences Journal, 31(7), 1237–1247.

Fouzia, A., Allaoua, S., Hafsa, C.S., & Mostefa, G. (2015). Plant growth promoting and antagonistic traits of indigenous fluorescent Pseudomonas spp. isolated from wheat rhizosphere and A. halimus endosphere. European Scientific Journal, 11(24), 129–148.

Hanudin, Nuryani, W., Silvia, E., Yusuf, & Marwoto, B. (2011). Biopestisida organik berbahan aktif Bacillus subtilis dan Pseudomonas fluorescens untuk mengendalikan penyakit layu Fusarium pada anyelir. J. Hort., 21(2), 152–163.

Hartati, S.Y. (2013). Efikasi formula fungisida nabati terhadap penyakit bercak daun jahe Phyllosticta sp. Bul. Littro, 24(1), 42-48.

Jain, K.M, Hessin, M.H., Achmadi, A., Dahon, N.H., Kimsuan, T., & Hamdan, H.S. (2011). Determination of total phenol, condensed tannin and flavonoid contents and antioxidant activity of Uncaria gambiir extracts. Majalah Farmasi Indonesia, 22(1), 50–59.

Janisiewicz, W.J., Tworkoski, T.J., & Sarer, C. (2000). Characterizing the mechanism of biological control of post harvest disease on fruits with a simple method to study competition for simple method to study competition for nutrients. Phytopathology, 90, 1196–1200.

Janousek., C.N., Lorber, J.D., & Gubler, W.D. (2009). Combination and rotation of bacterial antagonists to control powdery mildew on pumpkin. Journal of Plant Disease and Protection, 16(6), 260-262.

Kaewchai, S., & Soytong, K. (2010). Application of biofungicides against Rigidoporus microporus causing white root disease of rubber trees. Journal of Agricultural Technology, 6(2), 349–363.

Karimi, K., Amini, J., Harighi, B., & Bahramnejad, B. (2012). Evaluation of biocontrol potential of Pseudomonas and Bacillus spp. against Fusarium wilt of chickpea. AJCS, 6(4), 695–703.

Knobloch, K.A., Paul, B., Ilber, H., Weigand, & Weil, W. (1989). Antibacterial and antifungal properties of essential oil components. J. Ess-Oil, 1, 119-128.

Kusvianti, D., Widodo, & Prijono, D. (2014). Pengendalian penyakit busuk pangkal batang lada dengan ekstrak pinang, gambir, sirih dan kapur sirih. Jurnal Fitopatologi Indonesia. 10(4), 103–111.

Maharina, K.E., Aini, L.Q., & Wardiyati, T. (2014). Aplikasi agens hayati dan bahan nabati sebagai pengendalian layu bakteri (Ralstonia solanaceraum) pada budidaya tanaman tomat. Jurnal Produksi Tanaman, 1(6), 506–513.

Maleki, M., Mokhtarnejad, L., & Mostafaee, S. (2011). Screening of rhizobacteria for biological control of cucumber root and crown rot caused by Phytophthora drechsleri. Plant Pathol. J., 27(1), 78−84.

Manurung, L., Lubis, Marheni & Dalimunthe, C.I. (2015). Pengujian berbagai jenis bahan aktif terhadap penyakit jamur akar putih (JAP) (Rigidoporus microporus [Swartz: Fr.]) di areal tanpa olah tanah (TOT). Jurnal Online Agroekoteknologi, 3(1), 168-178.

Manjunatha, H., Naik, M.K., Patil, M.B., Lokesha, R., & Vasudevan, S.N. (2012). Isolation and characterization of native fluorescent Pseudomonads and antagonistic activity against major plant pathogens. Karnataka J.Agric.Sci., 25(3), 346–349.

Nasrun. (1997). Pengujian ekstrak daun serai wangi terhadap Scelrotium rolfsii penyebab penyakit busuk batang tanaman cabai. Kongres Nasional ke XIV dan Seminar Ilmiah PFI, di Palembang.

Nasrun, Christanti, Arwiyanto, T., & Mariska, I. (2005). Pengendalian penyakit layu bakteri nilam menggunakan Pseudomonas fluorescent. Jurnal Penelitian Tanaman Industri, 11(1), 19-24.

Nasrun, Christanti, Arwiyanto, T., & Mariska, I. (2007). Karakteristik fisiologis Ralstonia solanacearum penyebab penyakit layu bakteri nilam. Jurnal Penelitian Tanaman Industri, 13(2), 43-48.

Pulungan, M.H., Lubis, L., Zahara, F., & Fairuzah, Z. (2014). Uji efektifitas Trichoderma harzianum dengan formulasi granular ragi untuk mengendalikan penyakit jamur akar putih (Rigidoporus microporus [Swartz:fr.] Van Ov) pada tanaman karet di pembibitan. Jurnal On Line Agroekoteknologi, 2(2), 497-512.

Reddy, K.R.N., Reddy, C.S., & Muralidharan, K. (2009). Potential of botanical and biocontrol agents on growth and aflatoxin production by Aspergillus flavus infecting rice grains. Food Control, 20, 173–178.

Raupach, G.S., & Kloepper, J.W. (1998). Mixtures of plant growth-promoting rhizobacteria enhance biological control of multiple cucumber pathogens. Phytopathology, 88, 1158–1164.

Rhouma, A., Bouri, M., Boubaker, A., & Nesme, X.X. (2008). Potential effect of rhizobacteria in the management of crown gall disease caused by Agrobacterium tumefaciens Biovar 1. Journal of Plant Pathology, 90(3), 517−526.

Semangun, H. (1999). Penyakit-penyakit tanaman perkebunan di Indonesia. Yogyakarta: Gadjah Mada Press.

Sibarani, F.M. (2008). Uji efektifitas beberapa pestisida nabati untuk mengendalikan penyakit antraknosa (Colletotrichum capsici) pada tanaman cabai (Capsicum annum) di lapangan (Tesis S1, Fakultas Pertanian, Universitas Sumatera Utara Medan, Medan).

Silvia, Y.E., Nuryani, W., Djatnika, I., Hanudin, Suhardi, & Winarto, B. (2012). Potensi beberapa fungisida nabati dalam mengendalikan karat putih (Puccinia horana Henn) dan perbaikan mutu krisan. J. Hort., 22(4), 385–391.

Singh, H., Alsamarai, G., & Syarhabil, M. (2012). Performance of botanical pesticides to control post-harvest fungi in citrus. International Journal of Scientific & Engneering Research, 3(4), 1−4.

Srivastava, S.R. (2007). Screening for antifungal activity of pseudomonas fluorescens against phytopathogenic fungi. The Internet Journal of Microbiology, 5(2), 1–6.

Sutariati, G.A.K., & Wahab, A. (2010). Isolasi dan uji kemampuan Rizobakteri indigenous sebagai agensia hayati pengendali hayati penyakit pada tanaman cabai. J. Hort., 20(1), 86–95.

Thangavelu, R., & Gopi, M. (2015). Field suppression of Fusarium wilt disease in banana by combined application of native endophytic and rhizospheric bacterial isolates possessing multiple functions. Phytopathologia Mediterranea, 54(2), 241−252.

Tombe, M. (2008). Pemanfaatan pestisida nabati fungisida nabati dan agensia hayati untuk mengendalikan penyakit busuk jamur akar putih pada jambu mete. Bul. Littro, XIX(1), 68−77.

Tombe, M., Pangeran, D., & Haryani, T.S. (2012). Keefektifan formula minyak cengkeh dan seraiwangi terhadap Fusarium oxysporum f.sp. vanilae penyebab busuk batang vanili. Jurnal Littri, 18(4), 143−150.

Toua, D., Benchabane, M., Bensaid, F., & Bakour, R. (2013). Evaluation of Pseudomonas fluorescens for the biocontrol of Fusarium wilt in tomato and flax. African Journal of Microbiology Research, 7(58), 5449−5458.

Yildiz, H.N., Handan, H., Altino, & Dikilitas, M. (2012). Screening of Rhizobacteria against Fusarium oxysporum f.sp. melongenae the causal agent of wilt disease of eggplant. African Journal of Microbiology Research, 6(5), 3700−3706.

Yusuf, S., Nuryani, W., Djatnika, I., Hanudin, & Winarto, B. (2012). Potensi beberapa fungisida nabati dalam mengendalikan karat putih (Puccinia horiana Henn) dan perbaikan mutu krisan. J.Hort., 22(4), 385–391.




DOI: http://dx.doi.org/10.21082/jtidp.v2n2.2015.p61-68

Refbacks

  • There are currently no refbacks.




Copyright (c) 2015 Jurnal Tanaman Industri dan Penyegar

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.


 Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.


 

P-ISSN: 2356-1297
E-ISSN: 2528-7222
Accredited No.30/E/KPT/2018 on Oktober 24, 2018 by Ministry of Research, Technology and Higher Education of the Republic of Indonesia

                    


Jurnal Tanaman Industri dan Penyegar (JTIDP) Editorial Office :

Indonesian Industrial and Beverage Crops Research Institute
Jl. Raya Pakuwon Km. 2, Parungkuda, Sukabumi 43357 Jawa Barat Indonesia
Telp : (0266) 6542181
Fax : (0266) 6542087
Email : jtidp@litbang.pertanian.go.iduppublikasi@gmail.com
Website : http://balittri.litbang.pertanian.go.id



View My Stats