Evaluasi Bakteri Endofit untuk Pengendalian Nematoda Pratylenchus coffeae pada Tanaman Kopi

Rita Harni, Khaerati Khaerati

Abstract


Bakteri endofit adalah bakteri yang hidup dalam jaringan tanaman dan memberikan efek yang baik pada tanaman, dapat diisolasi dari akar, batang, daun, dan buah. Penelitian isolasi, seleksi, dan potensi bakteri endofit untuk mengendalikan nematoda Pratylenchus coffeae pada tanaman kopi telah dilakukan di Laboratorium dan Rumah Kaca Balai Penelitian Tanaman Industri dan Penyegar dari bulan Januari sampai Desember 2012. Tujuan penelitian adalah mengevaluasi isolat bakteri endofit yang potensial untuk mengendalikan nematoda pada tanaman kopi. Bakteri endofit diisolasi dari akar pertanaman kopi dari daerah Jawa Barat (KP. Pakuwon, Sukabumi, Garut, dan Pengalengan) dan Lampung (KP. Natar, KP. Cahaya Negeri, dan Liwa) menggunakan metode sterilisasi permukaan. Selanjutnya bakteri endofit diseleksi antagonismenya terhadap nematoda dan kemampuan memicu pertumbuhan tanaman. Hasil isolasi bakteri endofit dari akar kopi diperoleh 442 isolat dengan kerapatan populasi bakteri endofit 5x103–5,77x106 cfu/g berat basah akar. Dari 422 isolat yang diuji, 50 isolat (12,3%) di antaranya adalah isolat yang antagonis, 60 isolat (14,21%) terbukti dapat meningkatkan pertumbuhan tanaman. Hasil pengujian in vitro dan in vivo di rumah kaca diperoleh 3 isolat yang potensial menekan nematoda P. coffeae dan meningkatkan pertumbuhan tanaman kopi, yaitu PG132, PG76, dan LW15.


Kata Kunci: Kopi, isolasi, seleksi, potensi, bakteri endofit, nematoda, Pratylenchus coffeae

Endophytic bacteria are bacteria that live inside plant tissues and give a good effect on the plant, and can be isolated from the roots, stems, leaves, and fruit. Isolation, selection and potential of endophytic bacteria to control nematodes (Pratylenchus coffeae) on coffee plant had been carried out in the Laboratory and Greenhouse of Research Institute for Industrial and Beverage Crops from January to December 2012. The objectives of the study was to evaluate the potential of endophytic bacterial isolates to control nematodes in coffee plants. Endophytic bacteria were isolated from coffee root crops samples from several areas in West Java (KP. Pakuwon, Sukabumi, Garut, Pengalengan) and Lampung (KP. Natar, KP. Cahaya Negeri and Liwa). Furthermore, the isolates were selected their antagonistic activities and plant growth coffeae plant. A total of 442 isolates endophytic bacteria was obtained from coffee root with a population density of 5x103–5.77x106 cfu/g of fresh weight roots, as many as 50 (12.3%) isolates performed antagonis on nematodes, 60 isolat (14.21%) isolates stimulated the growth of coffeae plant. Result in vitro and in vivo test, there were 3 potential endophytic bacterial isolates, namely PG132, PG76, and LW15, effective to control P. coffeae and increase the coffee growth.


Keywords


Coffee; isolation; selection; potential; endophytic bacteria; nematode; Pratylenchus coffeae

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DOI: http://dx.doi.org/10.21082/jtidp.v4n2.2013.p109-116

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 Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.


 

P-ISSN: 2356-1297
E-ISSN: 2528-7222
Accredited No.30/E/KPT/2018 on Oktober 24, 2018 by Ministry of Research, Technology and Higher Education of the Republic of Indonesia

                    


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