Effect of Trichoderma spp. Secondary Metabolites and Botanical Fungicide to Control VSD Disease in Cacao

Rita Harni, Widi Amaria, Anis Herliyati Mahsunah, Irwan Lakani


Vascular streak dieback (VSD) caused by Ceratobasidium theobromae is a major disease in cacao which results in yield losses up to 45%. Controlling VSD using secondary metabolites and botanical fungicide is expected to reduce disease attacks because secondary metabolites contain antibiotics, enzymes, and toxins that can control the disease and botanical fungicide that are antifungal. The research aimed to determine the effect of Trichoderma spp. secondary metabolites and botanical fungicide to control VSD in cacao. The research was conducted in the Integrated Laboratory, IIBCRI, Sukabumi, Biotechnology Laboratory, BPPT, Serpong and farmer gardens in Rahmat Village, Palolo District, Sigi Regency, Central Sulawesi, from March to December 2017. The research used a randomized block design of 7 treatments with 3 replications; each replication consists of 10 plants. The treatments were: (1) Trichoderma virens LP1, (2) T. amazonicum LP3, (3) botanical fungicide, (4) T. virens LP1 + botanical fungicide, (5) T. amazonicum LP3 + botanical fungicide, (6) chemical fungicide (for comparison), (7) control (without application). Variables observed were the symptoms of the attack, severity and disease progression, and production. The results showed that Trichoderma spp. secondary metabolites suppress VSD disease severity and increase cacao production up to 33.97%–61.34%, either in a single form or in combination with botanical fungicide. T. virens LP1 secondary metabolite showed the highest suppression of VSD disease (54.18%), followed by the combination of T. virens LP1 + botanical fungicide (47.64%), better than chemical fungicide (30.89%). Thus, Trichoderma spp. secondary metabolites can be used as a VSD control technology.


Botanical fungicide; cacao; secondary metabolite; Trichoderma spp.; VSD

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Anita-Sari, I., & Susilo, A. W. (2013). Investigation of different characters of stomata on three cocoa clones with resistance level difference to VSD (vascular streak dieback) diseases. Journal of Agriculture Science and Technology, 3(9A), 703.

Deng, J., Li, W., Peng, X. L., & Hao, X. H. (2013). Study on the potential of antifungal activity of essential oils against fungal pathogens of fruits and vegetables. Journal of Chemical and Pharmaceutical Research, 5(12), 443–446.

Druzhinina, I. S., Seidl-Seiboth, V., Herrera-Estrella, A., Horwitz, B. A., Kenerley, C. M., Monte, E., … Kubicek, C. P. (2011). Trichoderma: The genomics of opportunistic success. Nature Reviews Microbiology, 9(10), 749–759. doi:10.1038/nrmicro2637

Dubey, S. C., Tripathi, A., Dureja, P., & Grover, A. (2011). Characterization of secondary metabolites and enzymes produced by Trichoderma species and their efficacy against plant pathogenic fungi. Indian Journal of Agricultural Sciences, 81(5), 455–461.

El-Zemiti, S. R., & Ahmed, S. M. (2005). Antifungal activity of some essential oils and their major chemical constituents against some phytopathogenic fungi. J. Pest. & Environ. Sci., 13(1), 61–72.

Guest, D., & Keane, P. (2007). Vascular-Streak dieback: A new encounter disease of cacao in Papua New Guinea and Southeast Asia caused by the obligate basidiomycete Oncobasidium theobromae. Phytopathology, 97(12), 1654–1657. doi:10.1094/PHYTO-97-12-1654

Halimah, & Sri-Sukamto. (2007). Intensitas penyakit vascular streak dieback pada sejumlah klon kakao koleksi Pusat Penelitian Kopi dan Kakao Indonesia. Pelita Perkebunan, 23, 118–128.

Harni, R., Amaria, W., Syafaruddin, & Mahsunah, H. (2017). Potensi metabolit sekunder Trichoderma spp . untuk mengendalikan penyakit vascular streak dieback (VSD) pada bibit kakao. Jurnal Tanaman Industri dan Penyegar, 4(2), 57–66. doi: http://dx.doi.org/10.21082/jtidp.v4n2.2017.p57-66

Harni, R., & Baharudin. (2014). Keefektifan minyak cengkeh, serai wangi, dan ekstrak bawang putih terhadap penyakit vascular streak dieback (Ceratobasidium theobromae) pada kakao. Jurnal Tanaman Industri dan Penyegar, 1(3), 167–174. doi: http://dx.doi.org/10.21082/jtidp.v1n3.2014.p167-174

Harni, R., & Khaerati. (2013). Potensi beberapa fungisida nabati untuk mengendalikan penyakit vascular streak dieback (VSD) pada bibit kakao. In Seminar dan Kongres PFI XXIII. Padang.

Harni, R., Taufiq, E., & Amaria, W. (2014). Pengaruh formula fungisida nabati minyak cengkeh dan serai wangi terhadap penyakit busuk buah kakao. Jurnal Tanaman Industri dan Penyegar, 1(1), 41–48. doi:http://dx.doi.org/10.21082/jtidp.v1n1.2014.p41-48

Howell, C. R. (2003). Mechanisms employed by Trichoderma species in the biological control of plant diseases: The history and evolution of current concepts. Plant Disease, 87. doi:10.1094/PDIS.2003.87.1.4

Karmawati, E., Mahmud, Z., Syakir, M., Ardana, I. K., Munarso, J., & Rubiyo. (2010). Budidaya dan Pasca Panen Kakao. Bogor: Badan Litbang Pertanian.

McMahon, P., & Purwantara, A. (2016). Vascular streak dieback (Ceratobasidium theobromae): History and Biology. In B. A. Bailey & L. W. Meinhardt (Eds.), Cacao diseases. A history of old enemies and new encounters (pp. 307–336). Springers. doi:10.1007/978-3-319-24789-2_9

Nakahara, K., Alzoreky, N. S., Yoshihashi, T., Nguyen, H. T. T., & Trakoontivakom, G. (2003). Chemical composition and antifungal activity of essential oil from Cymbopogon narduse. JARQ, 37(4), 249–252.

Noveriza, R., Trisno, J., Rahma, H., Yuliani, S., Reflin, & Martinius. (2018). Effectiveness of several dosage formula of oil and nano emulsion of citronella against vascular streak dieback (VSD) disease on cocoa. In IOP Conference Series: Earth and Environmental Science, 122, 0–8. doi:10.1088/1755-1315/122/1/012028

Samuels, G. J., Ismaiel, A., Rosmana, A., Junaid, M., Guest, D., Mcmahon, P., … Cubeta, M. A. (2012). Vascular streak dieback of cacao in Southeast Asia and Melanesia: In planta detection of the pathogen and a new taxonomy. Fungal Biology, 116(1), 11–23. doi:10.1016/j.funbio.2011.07.009

Soesanto, L. (2013). Pengendalian hayati penyakit tanaman. Jakarta: PT. Raja Grafindo Persada.

Strange, R. N. (2003). Introduction to plant pathology. New York.

Vinale, F., Marra, R., Scala, F., Ghisalberti, E. L., Lorito, M., & Sivasithamparam, K. (2006). Major secondary metabolites produced by two commercial Trichoderma strains active against different phytopathogens. Letters in Applied Microbiology, 43(2), 143–148. doi:10.1111/j.1472-765X.2006.01939.x

Vinale, F., Sivasithamparam, K., Ghisalberti, E. L., Woo, S. L., Nigro, M., Marra, R., … Lorito, M. (2014). Trichoderma secondary metabolites active on plants and fungal pathogens. The Open Mycology Journal, 8(1), 127–139. doi:10.2174/1874437001408010127

Zeilinger, S., Gruber, S., Bansal, R., & Mukherjee, P. K. (2016). Secondary metabolism in Trichoderma - Chemistry meets genomics. Fungal Biology Reviews, 30(2), 74–90. doi:10.1016/j.fbr.2016.05.001

DOI: http://dx.doi.org/10.21082/jtidp.v6n3.2019.p109-118


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